Cell separation methods and applications
Cell separation methods and applications
复制标题
细胞分离方法及应用
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
Andreas Radbruch
中科院分区：
文献类型：
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作者：
D. Recktenwald;Andreas Radbruch
背景：历史和有用的方法预选和准备规模排序。物理分离方法：在人类造血祖细胞的处理中使用胶体硅梯度密度梯度分离的新方法离心洗脱-细胞生物学免疫学和血液学中的一种强大的分离技术。基于抗体的方法：使用聚苯乙烯免疫亲和装置的细胞疗法的分离、激活、扩增和基因转导-技术、临床发展和未来方向高密度颗粒-一种新型、高效的细胞分离技术抗体和补体介导的细胞分离。磁性方法：磁性细胞分选采用胶体超顺磁性颗粒，免疫磁性细胞分离采用抗体和超顺磁性微球自由流动磁电泳-通过磁性偏移和聚焦对细胞和细胞器进行连续免疫磁性分选。流式细胞术：利用流式细胞术进行细胞分离。特殊应用：利用表面标记选择转染细胞CD34细胞分选和富集-在血库和移植中的应用。附录：细胞分离的细胞特性CD抗原的名称。术语表。
Background: historical and useful methods of preselection and preparative scale sorting. Physical methods of separation: new approaches in density gradient separation using colloidal silica gradients in the processing of human haematopoietic progenitor cells centrifugal elutriation - a powerful separation technique in cell biology immunology, and haematology. Antibody-based methods: isolation, activation, expansion, and gene transduction of cell-based therapeutics using polystyrene immunoaffinity devices the CEPRATER SC system - technology, clinical development, and future directions high density particles - a novel, highly efficient cell separation technology antibody and complement mediated cell separation. Magnetic methods: magnetic cell sorting with colloidal superparamagnetic particles immunomagnetic cell separation using antibodies and superparamagnetic microspheres free flow magnetophoresis - continuous immunomagnetic sorting of cells and organelles by magnetic deviation and focusing. Flow cytometry: cell separation using flow cytometric cell sorting. Special applications: employing surface markers for the selection of transfected cells CD34 cell sorting and enrichment - applications in blood banking and transplantation. Appendices: cellular properties for cell separation CD antigen designations. Glossary.