Pancreatic Carcinoma Cell Lines Reflect Frequency and Variability of Cancer Stem Cell Markers in Clinical Tissue
Pancreatic Carcinoma Cell Lines Reflect Frequency and Variability of Cancer Stem Cell Markers in Clinical Tissue
复制标题
DOI：
10.1159/000341669
复制
发表时间：
2012-01-01
影响因子：
1.6
通讯作者：
Habermann, J. K.
中科院分区：
文献类型：
--
作者：
Buenger, S.;Barow, M.;Habermann, J. K.
背景：胰腺癌是最致命的恶性肿瘤之一，治疗选择不足，预后差。癌症干细胞（CSC）被认为是负责进展和治疗抗性。我们通过分析胰腺细胞系在多大程度上包含CSC群体以及与临床组织相比这些群体的代表性来研究胰腺细胞系用于CSC研究的潜力。方法：采用流式细胞仪检测6株胰腺癌细胞系中CD326、CD133、CD44、CD24、CXCR 4和ABCG 2的表达。随后，通过免疫组织化学评价70个原代胰腺组织的CD 326、CD 133和CD 44。结果：除一个细胞系外，所有细胞系在整个生物学重复中均显示稳定的表达模式。CD44在临床组织中的标记表达以50%的敏感性和80%的特异性区分正常患者和胰腺癌患者，以69%的敏感性和100%的特异性区分转移性和非转移性癌。结论：我们的研究结果表明，CD44表达升高，恶性和胰腺组织转移之间的联系。此外，单个胰腺细胞系显示出大量具有CSC特性的细胞，这与在原代组织中检测到的患者间变异性相当。因此，这些胰腺癌细胞系可用于迫切需要的药理学CSC体外研究。版权所有（C）2012 S. Karger AG，巴塞尔
Background: Pancreatic cancer is one of the most deadly malignancies with insufficient therapeutic options and poor outcome. Cancer stem cells (CSCs) are thought to be responsible for progression and therapy resistance. We investigated the potential of pancreatic cell lines for CSC research by analyzing to what extent they contain CSC populations and how representative these are compared to clinical tissue. Methods: Six pancreatic cancer cell lines were analyzed by flow cytometry for CD326, CD133, CD44, CD24, CXCR4 and ABCG2. Subsequently, 70 primary pancreatic tissues were evaluated for CD326, CD133 and CD44 by immunohistochemistry. Results: All the cell lines but one showed a stable expression pattern throughout biological replicates. Marker expression in clinical tissue of CD44 distinguished normal patients from pancreatic carcinoma patients with a sensitivity of 50% at 80% specificity and metastasized from nonmetastasized carcinomas with 69% sensitivity at 100% specificity. Conclusions: Our results indicate a link between elevated CD44 expression, malignancy and metastasis of pancreatic tissue. Furthermore, individual pancreatic cell lines show a substantial amount of cells with CSC properties which is comparable with interpatient variability detected in primary tissue. These pancreatic cancer cell lines could thus serve for urgently needed pharmacological CSC in vitro research. Copyright (C) 2012 S. Karger AG, Basel