Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides.
Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides.
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DOI：
10.1039/c2cc35028a
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发表时间：
2012-09
影响因子：
4.9
通讯作者：
Vanessa Siegmund;Tobias Santner;R. Micura;A. Marx
中科院分区：
文献类型：
--
作者：
Vanessa Siegmund;Tobias Santner;R. Micura;A. Marx
我们提出了一种基于荧光读数的筛选试验，用于接受2 '-修饰核苷酸的T7 RNA聚合酶变体的定向进化。通过使用该筛选，我们能够鉴定出具有增加的2 '-甲基硒基-2'-脱氧尿苷5 '-三磷酸接受性的T7 RNA聚合酶突变体。
We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2'-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2'-methylseleno-2'-deoxyuridine 5'-triphosphate.