Functional diversity, conservation, and convergence in the evolution of the alpha-, beta-, and gamma-carbonic anhydrase gene families
Functional diversity, conservation, and convergence in the evolution of the alpha-, beta-, and gamma-carbonic anhydrase gene families
复制标题
DOI：
10.1006/mpev.1996.0006
复制
发表时间：
1996-02-01
影响因子：
4.1
通讯作者：
Tashian, RE
中科院分区：
文献类型：
--
作者：
HewettEmmett, D;Tashian, RE
碳酸酐酶(CA)高效催化二氧化碳的可逆水合反应，这一反应存在于动物、植物、古细菌和真细菌的许多不同的生理过程中。我们研究了由三个独立的CA基因家族(α-CA、β-CA和Gamma-CA)成员编码的CA的进化历史和功能收敛。令人惊讶的是，6种确定功能和组织表达的哺乳动物α-CA同工酶的进化速度比4种未知功能的哺乳动物α-CA相关蛋白进化得更快。我们已经确定并包括了几个存在于序列数据库中的以前未被识别的CA同源物，其中许多是基因组计划测序和表达的cDNA研究的成果。我们研究了α-CA活性中心的演化以及推测的β-CA和伽马-CA活性中心。通过对α-CA内含子位置的分析，我们发现了对“内含子延迟”假说的支持。认为α-CA仅限于动物界和植物绿藻(衣藻)，β-CA限于植物和真细菌，伽马-CA限于古细菌和真细菌的观点正在瓦解。植物拟南芥拥有这三个家族的同源物。(C)1996年学术出版社。
The carbonic anhydrases (CA) catalyze with high efficiency the reversible hydration of carbon dioxide, a reaction underlying many diverse physiological processes in animals, plants, archaebacteria, and eubacteria. We examined the evolutionary history and functional convergence of the CAs encoded by members of three independent CA gene families (alpha-CA, beta-CA and gamma-CA). Surprisingly, the six mammalian alpha-CA isozymes of defined function and tissue expression are evolving more rapidly than four mammalian alpha-CA-related proteins of unknown function. We have identified and included several previously unrecognized CA homologues present in the sequence databases, many of which are the fruits of genome project sequencing and expressed cDNA studies. We examined alpha-CA active site evolution and the putative beta-CA and gamma-CA active sites. We found support for the ''introns late'' hypothesis by analysis of alpha-CA intron locations. The view that alpha-CAs would be restricted to the animal kingdom and plant green algae (Chlamydomonas), the beta-CAs to plants and eubacteria, and the gamma-CAs to archaebacteria and eubacteria is breaking down. The plant Arabidopsis has homologues of all three families. (C) 1996 Academic Press, Inc.