Full-length HERV-H elements with env SU open reading frames in the human genome
Full-length HERV-H elements with env SU open reading frames in the human genome
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DOI：
10.1089/088922202760019383
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发表时间：
2002-06-10
影响因子：
1.5
通讯作者：
Blomberg, J
中科院分区：
文献类型：
--
作者：
Jern, P;Lindeskog, M;Blomberg, J
人类内源性逆转录病毒（HERV）估计占人类基因组的至少1%。使用HERV-H env SU序列（HERV-H19）筛选高通量（htgs）和非冗余（nr）数据库中的其他HERV-H SU开放阅读框（ORF），从而筛选可能的功能蛋白。使用PCR与引物来自HERV-H19 SU，我们还获得了几个新的序列与ORF从人类DNA样品。在系统发育分析中，含有ORF的序列与来自染色体1和2的HERV-H序列聚类。含SU ORF和不含SU ORF的元件在5'和3'长末端重复序列（LTR）之间具有大约相同的差异（约4%），表明整合时间相似。SU ORF序列有中等数量的同义突变与非同义突变，这表明HERV-H SU ORF的维持选择。
Human endogenous retroviruses (HERVs) are estimated to represent at least 1% of the human genome. An HERV-H env SU sequence (HERV-H19) was used to screen the high-throughput (htgs) and nonredundant (nr) databases for other HERV-H SU open reading frames (ORFs) and thus possible functional proteins. Using PCR with primers derived from HERV-H19 SU, we also obtained several new sequences with ORFs from a human DNA sample. In a phylogenetic analysis, ORF-containing sequences clustered with HERV-H sequences from chromosomes 1 and 2. SU ORF- and non-SU ORF-containing elements had about the same difference between 5' and 3' long terminal repeats (LTRs) (about 4%), indicating a similar time of integration. SU ORF sequences had a moderately high number of synonymous-versus-nonsynonymous mutations, which indicates a selection for maintenance of the HERV-H SU ORFs.