Early in vivo and in vitro effects of amelogenin gene splice products on pulp cells
Early in vivo and in vitro effects of amelogenin gene splice products on pulp cells
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DOI：
10.1111/j.1600-0722.2006.00320.x
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发表时间：
2006-05-01
影响因子：
1.9
通讯作者：
Poliard, Anne
中科院分区：
文献类型：
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作者：
Lacerda-Pinheiro, Sally;Jegat, Nadege;Poliard, Anne
将重组釉原蛋白基因剪接产物A+4和A-4植入牙髓中，诱导修复细胞的募集、增殖和分化。我们的目的是调查发生在牙髓早熟事件后1天和3天单独或加载A+4或A-4琼脂糖珠植入。通过检测增殖细胞核抗原（PCNA）和RP 59观察增殖和细胞向牙/成骨表型的募集。在植入单独的珠或加载有A+4后，在第3天，牙髓细胞对骨桥蛋白（OP）呈中度免疫阳性，而标记在用A-4处理后呈强阳性。牙本质涎蛋白（DSP）标记未检测到。对成牙本质细胞和间充质祖细胞进行了平行的体外研究，以评估釉原蛋白肽对成牙本质细胞/成骨细胞/成软骨细胞分化途径中涉及的一系列标记基因表达的影响。总之，我们的研究结果表明，釉原蛋白肽A+4和A-4的“信号”作用可能会根据靶细胞的类型，分化阶段，治疗时间和釉原蛋白肽的类型（A+4或A-4）而有所不同。
Recombinant amelogenin gene splice products A+4 and A-4, implanted in the pulp, induce the recruitment, proliferation, and differentiation of reparative cells. Our aim was to investigate the precocious events occurring in the pulp 1 d and 3 d after implantation of agarose beads alone or loaded with A+4 or A-4. Proliferation and cell recruitment towards an odonto/osteogenic phenotype were visualized by detection of the proliferation cell nuclear antigen (PCNA) and RP59. After implantation of beads alone or loaded with A+4, at day 3, pulp cells were moderately immunopositive for osteopontin (OP), whereas labeling was strongly positive upon treatment with A-4. Dentin sialoprotein (DSP) labeling was not detectable. Parallel in vitro studies were carried out on odontoblastic and mesenchymal progenitor cells in order to evaluate the effect of the amelogenin peptides on the expression of a series of marker genes involved in the odontoblastic/osteogenic/chondrogenic differentiation pathways. Altogether, our results suggest that the 'signaling' effects of the amelogenin peptides A+4 and A-4 may differ according to the type of target cells, their stage of differentiation, the time of treatment, and the type of amelogenin peptide (A+4 or A-4).