Endocrine disruptor effect of perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) on porcine ovarian cell steroidogenesis
Endocrine disruptor effect of perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) on porcine ovarian cell steroidogenesis
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DOI：
10.1016/j.tiv.2017.09.030
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发表时间：
2018-02-01
影响因子：
3.2
通讯作者：
Ducolomb, Yvonne
中科院分区：
文献类型：
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作者：
Chaparro-Ortega, Andrea;Betancourt, Miguel;Ducolomb, Yvonne
以前对全氟辛烷磺酸和全氟辛酸的研究表明，它们除了引起改变和损害生殖健康外，还可作为内分泌干扰物;然而，产生这些疾病的生物机制尚不清楚。本研究的目的是分析全氟辛烷磺酸和全氟辛酸对猪卵泡膜和颗粒细胞体外类固醇分泌的影响，有或没有促性腺激素刺激。分离培养颗粒细胞和卵泡膜细胞。免疫细胞化学法检测细胞性质。化学发光法分析PFOS和PFOA对类固醇分泌的影响。在本研究中，当向卵泡膜和颗粒细胞施用全氟辛烷磺酸（0.12、1.2、12、120或240 μ M）或全氟辛酸（0.012、0.12、1.2、12或24 μ M）时，发现类固醇分泌发生变化。当卵泡膜和颗粒细胞分别用500纳克/毫升LH或500纳克/毫升FHS刺激，然后立即用1.2 μ M的全氟辛烷磺酸或全氟辛酸，全氟化合物抑制类固醇激素的分泌在两个刺激的细胞类型。结果表明，PFOS和PFOA作为内分泌干扰物作用于类固醇卵巢细胞，可能影响性类固醇的依赖功能。
Previous studies with perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) indicate that they act as endocrine disrupters, in addition to inducing alterations and damaging reproductive health; however, the biological mechanisms by which these disorders are produced are not yet understood. The aim of this study was to analyze the effect of PFOS and PFOA on in vitro steroidogenic secretion in porcine theca and granulosa cells, with or without gonadotropic stimulation. Granulosa and theca cells were isolated and cultured. Cell nature was performed by immunocytochemistry. PFOS and PFOA effect on steroid secretion was analyzed by chemiluminescence. In the present study, alterations in steroidogenic secretion were found when administering PFOS (0.12, 1.2,12, 120 or 240 mu M) or PFOA (0.012, 0.12, 1.2, 12 or 24 mu M) to theca and granulosa cells. When theca and granulosa cells were stimulated with 500 ng/mL LH or 500 ng/mL FHS, respectively and immediately followed with 1.2 mu M of PFOS or PFOA, the perfluorinated compounds inhibited the secretion of steroid hormones in both stimulated cell types. The results indicate that PFOS and PFOA act on steroidogenic ovarian cells as endocrine disrupters, which could affect the dependent functions of sexual steroids.