Nano-magnetic aptamer sensor incorporating AND logic recognition-launched hybridization chain reaction for organ origin identification of circulating tumor cells
Nano-magnetic aptamer sensor incorporating AND logic recognition-launched hybridization chain reaction for organ origin identification of circulating tumor cells
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DOI：
10.1016/j.nantod.2023.101817
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发表时间：
2023-04
期刊：
影响因子：
17.4
通讯作者：
Yujia Zhang;Yue Yu;Ke Kang;Xuekang Wang;Nanhang Zhu;Xingyou Wang;Qiangying Yi;Yao Wu
中科院分区：
文献类型：
--
作者：
Yujia Zhang;Yue Yu;Ke Kang;Xuekang Wang;Nanhang Zhu;Xingyou Wang;Qiangying Yi;Yao Wu
循环肿瘤细胞（Circulating tumor cells，CTC）是肿瘤早期诊断和病情监测的有效生物标志物，但目前缺乏简单有效的策略实现CTC上多种标志物的检测，同时富集，特别是器官来源的鉴定，限制了其广泛的临床应用。在这里，我们提出了一种纳米磁性适体传感器平台，以捕获广谱上皮细胞CTC，并同时确定其多个器官的起源与逻辑推理启动的杂交链反应。该平台表现出对上皮细胞CTC的出色捕获和富集能力，并显示出精确识别其器官来源标志物（肝脏的GPC 3和肺部的TTF 1）的能力，甚至能够将转移性癌症追踪到相关器官。所开发的纳米磁性适体传感器平台为CTC的多标志物精确检测提供了可行的策略，并为癌症的精确治疗提供了有前途的辅助工具。
Circulating tumor cells (CTCs) are effective bio-markers for early diagnosis and disease monitoring of cancer, but there is lack of simple and effective strategy to achieve the detection of multiple markers on CTCs while enriching, especially the identification of organ origin, limits their broad clinical applications. Here we present a nano-magnetic aptamer sensor platform to capture broad spectrum of epithelial CTCs and simultaneously identify their multiple organ origins by AND logic recognition-launched hybridization chain reaction. The platform exhibited excellent capture and enrichment capabilities for epithelial CTCs, and showed the ability to precisely identify their organ origin markers (GPC3 for liver and TTF1 for lung), even able to trace metastatic cancer to related organs. The developed nano-magnetic aptamer sensor platform provides a feasible strategy for multi-marker accurate detection of CTCs and a promising auxiliary tool for the precise treatment of cancer.