Usnic acid, a natural antimicrobial agent able to inhibit bacterial biofilm formation on polymer surfaces
Usnic acid, a natural antimicrobial agent able to inhibit bacterial biofilm formation on polymer surfaces
复制标题
DOI：
10.1128/aac.48.11.4360-4365.2004
复制
发表时间：
2004-11-01
影响因子：
4.9
通讯作者：
Stoodley, P
中科院分区：
文献类型：
--
作者：
Francolini, I;Norris, P;Stoodley, P
在现代医学中，人工设备被用来修复或替换身体受损部分，输送药物，并监测危重病人的病情。然而，人造表面往往容易受到细菌和真菌的侵袭。一旦微生物附着在表面，它们就可以形成生物膜，导致高度耐药的局部或系统感染。此时，有证据表明，次生地衣代谢产物(+)-松萝酸对包括金黄色葡萄球菌、粪肠球菌和粪肠球菌在内的一些浮游革兰氏阳性细菌具有抗菌活性。由于地衣是表面附着的群落，产生抗生素，包括松萝酸，以保护自己免受其他细菌的侵袭，我们假设松萝酸的作用方式可能用于控制医用生物膜。我们将(+)-松萝酸负载到改性聚氨酯中，并通过图像分析定量评估了(+)-松萝酸在层流条件下对金黄色葡萄球菌和铜绿假单胞菌生物膜形成的抑制能力。(+)-尿酸聚合物不能抑制金黄色葡萄球菌细胞的初始附着，但杀死附着的细胞会导致生物膜的抑制。有趣的是，尽管(+)-松萝酸负载聚合物表面确实形成了铜绿假单胞菌生物膜，但生物膜的形态发生了改变，这可能表明(+)-松萝酸干扰了信号通路。
In modern medicine, artificial devices are used for repair or replacement of damaged parts of the body, delivery of drugs, and monitoring the status of critically ill patients. However, artificial surfaces are often susceptible to colonization by bacteria and fungi. Once microorganisms have adhered to the surface, they can form biofilms, resulting in highly resistant local or systemic infections. At this time, the evidence suggests that (+)-usnic acid, a secondary lichen metabolite, possesses antimicrobial activity against a number of planktonic gram-positive bacteria, including Staphylococcus aureus, Enterococcus faecalis, and Enterococcus faecium. Since lichens are surface-attached communities that produce antibiotics, including usnic acid, to protect themselves from colonization by other bacteria, we hypothesized that the mode of action of usnic acid may be utilized in the control of medical biofilms. We loaded (+)-usnic acid into modified polyurethane and quantitatively assessed the capacity of (+)-usnic acid to control biofilm formation by either S. aureus or Pseudomonas aeruginosa under laminar flow conditions by using image analysis. (+)-Usnic acid-loaded polymers did not inhibit the initial attachment of S. aureus cells, but killing the attached cells resulted in the inhibition of biofilm. Interestingly, although P. aeruginosa biofilms did form on the surface of (+)-usnic acid-loaded polymer, the morphology of the biofilm was altered, possibly indicating that (+)-usnic acid interfered with signaling pathways.