Streptococcal Pyrogenic Exotoxin F (SpeF) Causes Permeabilization of Lung Blood Vessels
Streptococcal Pyrogenic Exotoxin F (SpeF) Causes Permeabilization of Lung Blood Vessels
复制标题
链球菌热原性外毒素 F (SpeF) 导致肺血管通透化
作者：
M. Matsumoto;N. Ishikawa;M. Saito;K. Shibayama;T. Horii;Kumiko Sato;M. Ohta
急性呼吸窘迫综合征（ARDS）是化脓性链球菌引起的中毒性休克样综合征（TSLS）的典型并发症。本研究采用大鼠离体肺灌流模型，对TSLS的ARDS病因进行了初步探讨。从S.化脓菌分离物引起灌注肺重量的快速增加，表明血管透化。来自TSLS和咽炎患者的M 1型和3型分离株的6份样品显示出较强的透化活性，而来自其他M型分离株的制剂（尽管检查的分离株数量有限）为阴性。使用各种色谱柱通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳将活性物质纯化为单一条带，并测定N-末端氨基酸序列。所得的8个氨基酸序列与SpeF（促有丝分裂因子）相同。此外，用纯化的SpeF免疫制备的抗SpeF抗血清消除纯化条带的血管透化活性。该活性也被通过用衍生自公开的SpeF序列的合成肽免疫制备的抗血清中和。提示SpeF是导致肺血管通透性增高的主要原因，在链球菌引起的TSLS条件下足以导致ARDS的发生。化脓
ABSTRACT Acute respiration distress syndrome (ARDS) is a typical complication in toxic shock-like syndrome (TSLS) caused byStreptococcus pyogenes. An isolated perfused rat lung model was used to identify the causative agent of ARDS in TSLS in this study. Some crude preparations separated from the culture supernatants ofS. pyogenes isolates caused rapid increases in the weight of perfused lungs, indicating vascular permeabilization. Six samples from M type 1 and 3 isolates from TSLS and pharyngitis patients showed strong permeabilization activity, whereas preparations from isolates of other M types (although the number of isolates examined was limited) were negative. The active substance was purified to a single band by sodium dodecyl sulfate-polyacrylamide gel electrophoresis using various columns, and the N-terminal amino acid sequence was determined. The resultant sequence of eight amino acids was identical to SpeF (mitogenic factor). Moreover, the vascular permeabilization activity of the purified band was abolished with anti-SpeF antiserum prepared by immunizing with the purified SpeF. This activity was also neutralized by the antiserum prepared by immunizing with a synthetic peptide derived from the published SpeF sequence. These results suggested that streptococcal SpeF is a major cause of permeabilization of lung blood vessels and sufficient for the pathogenesis of ARDS under the conditions of TSLS caused by S. pyogenes.
DOI：
10.1073/pnas.90.16.7676
发表时间：
1993-08-15
影响因子：
11.1
作者：
KAPUR, V;MAJESKY, MW;MUSSER, JM
通讯作者：
MUSSER, JM