Multiplication and cryopreservation of yarrow (Achillea millefolium L., Asteraceae).
Multiplication and cryopreservation of yarrow (Achillea millefolium L., Asteraceae).
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发表时间：
2013-01
影响因子：
1.2
通讯作者：
M. Shatnawi
中科院分区：
文献类型：
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作者：
M. Shatnawi
千叶冬青属菊科，是约旦及其邻国的濒危药用植物。作为种子繁殖的一种替代方法，研究了A.千屈菜及其随后的生根被开发作为其体外保存的一种选择。在Murashige和Skoog琼脂培养基中添加0.9mgL-1的6-苄基氨基嘌呤（BAP），最多可获得5.9个芽/枝。试验了不同种类和浓度的生长素IBA（Indole-3-Butyric-Acid）、IAA（Indole-3-Acetic-Acid）和NAA（NaphthaleneAceticAcid）的效果。当IBA浓度为1.2mg L-1时，不定根数最高，为20.8条。70%的存活率时，生根外植体在体内驯化在等量的泥炭土和泥炭土。在离体条件下，A.在24±2°C下，在补充有不同浓度的蔗糖、葡萄糖或果糖的MS培养基上成功地将千叶芽储存长达32周。32周后，在附加3%蔗糖的培养基上，88.6%的芽成活。此外，85.3%的新梢能够在光照条件下重新生长。当茎尖在补充有0.4M山梨糖醇和0.1M蔗糖的培养基上预培养1天，随后用浓缩的植物玻璃化溶液2（PVS 2）加载茎尖20分钟，然后在液氮（LN）中储存之前在0°C下用PVS 2脱水60分钟时，成功地实现了通过玻璃化的超低温保存（80%再生长）。
Achillea millefolium belonging to the Astreaceae family is an endangered medicinal plant of Jordan and of its neighboring countries. As an alternative to seed propagation, an efficient micropropagation of A. millefolium and its subsequent rooting were developed as an option for its in vitro conservation. A maximum of 5.9 shoots per microshoot were obtained on Murashige and Skoog agar medium supplemented with 0.9 mg L -1 of 6Benzyl Amino Purine (BAP). The effect of different types and concentrations of auxins were tested, i.e. IBA (Indole-3-Butyric-Acid), IAA (Indole-3-Acetic-Acid) or Naphthalene Acetic Acid (NAA). Maximum root number (20.8 roots ex-plant -1 ) was obtained from media containing 1.2 mg L -1 of IBA. A survival of 70% was obtained when rooted explants were acclimatized in vivo in equal portions of perlite and peat soil. In vitro, A. millefolium shoots were successfully stored for up to 32 weeks on MS medium supplemented with different concentrations of either sucrose, glucose or fructose, at 24±2°C. After 32 weeks past, 88.6% of the shoots survived on the medium supplemented with 3% sucrose. Moreover, 85.3% of the shoots were able to re-grow when stored under light conditions. Cryopreservation through vitrification was successfully achieved (80% re-growth) when shoot tips precultured on a medium supplemented with 0.4M sorbitol and 0.1M sucrose for 1 day, followed by loading shoot tips with concentrated plant vitrification solution 2 (PVS2) for 20 minutes, then being dehydrated with PVS2 for 60 minutes at 0°C prior to storage in Liquid Nitrogen (LN).