The immunological activity of a deletion mutant of influenza virus haemagglutinin lacking the globular region
The immunological activity of a deletion mutant of influenza virus haemagglutinin lacking the globular region
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DOI：
10.1099/0022-1317-77-7-1483
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发表时间：
1996-07-01
影响因子：
3.8
通讯作者：
Isegawa, Y
中科院分区：
文献类型：
--
作者：
Sagawa, H;Ohshima, A;Isegawa, Y
缺失突变的流感病毒血凝素（HA;无头HA）缺乏的球状区域在CV-1细胞中表达，并检测与单克隆抗体，C179，它识别的构象表位在中间的HA的干区和中和所有的H1和H2亚型。编码无头HA的cDNA由流感病毒A/Okuda/S7（H2 N2）构建，其也用于选择C179。C179识别的构象表位即使在去除球状区域后也高度稳定。用无头HA免疫小鼠并用致死性流感病毒A/FM/1/47（H1N1）攻击，其存活率显著高于对照小鼠。无头HA具有诱导针对流感病毒感染的交叉保护的潜力。
A deletion mutant of influenza virus haemagglutinin (HA; headless HA) lacking the globular region was expressed in CV-1 cells and detected with a monoclonal antibody, C179, which recognizes a conformational epitope in the middle of the stem region of HA and neutralizes all H1 and H2 subtypes. The cDNA coding for the headless HA was constructed from influenza virus A/Okuda/S7 (H2N2), which was also used to select C179. The conformational epitope recognized by C179 was highly stable even after removal of the globular region. The survival rate of mice immunized with the headless HA and challenged with lethal influenza virus A/FM/1/47 (H1N1) was significantly higher than that of the control mice. The headless HA has the potential to induce cross-protection against influenza virus infection.