Anti-biofilm activity of A22 ((S-3,4-dichlorobenzyl) isothiourea hydrochloride) against Pseudomonas aeruginosa: Influence on biofilm formation, motility and bioadhesion
Anti-biofilm activity of A22 ((S-3,4-dichlorobenzyl) isothiourea hydrochloride) against Pseudomonas aeruginosa: Influence on biofilm formation, motility and bioadhesion
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DOI：
10.1016/j.micpath.2017.08.008
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发表时间：
2017-10-01
影响因子：
3.8
通讯作者：
Anraku de Campos, Marli Matiko
中科院分区：
文献类型：
--
作者：
Bonez, Pauline Cordenonsi;Rossi, Grazielle Guidolin;Anraku de Campos, Marli Matiko
细菌生物膜参与各种医疗感染，因此，了解相关微生物的粘附机制对于开发新的预防和控制策略至关重要。不同的方法已被用于在医疗保健环境中预防生物膜相关的感染，例如在医疗植入物中使用表面涂层剂。在此背景下，有必要探索具有抗生物膜活性的新化合物。因此，本研究首次评估了A22对生物和非生物表面上的铜绿假单胞菌PAO 1菌株和多重耐药临床分离株的生物膜的作用。A22作为细菌细胞壁的MreB蛋白的抑制剂，导致杆改变形状为球形形式。在这项工作中，亚抑制浓度的A22能够防止生物膜形成，原子力显微镜图像显示，A22在抑制聚乙烯表面粘附方面非常有效。铜绿假单胞菌PAO1对HeLa细胞具有较强的粘附能力，A22在4 h后可抑制其聚集。在亚抑制浓度下，A22显著改变了群集和抽搐运动。因此，通过改变细菌细胞的形状，可以影响许多特性，例如运动性、表面粘附和生物膜形成。A22是一种很有前途的新型抗菌剂或医用材料表面涂层剂。(C)2017爱思唯尔有限公司版权所有。
Bacterial biofilms are involved in various medical infections and for this reason it is of great importance understanding adhesion mechanisms of involved microorganisms is essential to develop new strategies of prevention and control. Different approaches have been used for preventing biofilm related infections in health care settings, such as use of surface coatings agents in medical implants. In this context, is necessary to explore new compounds with anti-biofilm activity. Thus, this study evaluated for the first time the action of A22 against biofilms of Pseudomonas aeruginosa PAO1 strain and multi-resistant clinical isolates on biotic and abiotic surfaces. A22 acts as inhibitor of the MreB protein of the bacterial cell wall, causing the rods to change shape to the coccoid form. In this work, A22 at subinhibitory concentrations was able to prevent biofilm formation, and atomic force microscopy images showed that A22 was highly effective in inhibiting adhesion on polyethylene surfaces. Pseudomonas aeruginosa PAO1 exhibited a strong ability to adhere to HeLa cells, and A22 inhibited the aggregation after 4 h of exposure. Swarming and twitching motilities were significantly altered by A22 at subinhibitory concentrations. Thus, by changing the shape of the bacterial cell, many properties can be affected, such as motility, surface adhesion and biofilm formation. This work presents A22 as a promising novel antibacterial or surface coating agent of medical materials. (C) 2017 Elsevier Ltd. All rights reserved.