Genes for biosynthesis of butenolide-like signalling molecules in Streptomyces ghanaensis, their role in moenomycin production.
Genes for biosynthesis of butenolide-like signalling molecules in Streptomyces ghanaensis, their role in moenomycin production.
复制标题
加纳链霉菌中丁烯酸内酯样信号分子生物合成的基因及其在莫诺霉素生产中的作用。
DOI：
10.1134/s1022795414060076
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发表时间：
2014
影响因子：
0.6
通讯作者：
Fedorenko,V
中科院分区：
文献类型：
--
作者：
Mutenko,H;Makitrinskyy,R;Tsypik,O;Walker,S;Ostash,B;Fedorenko,V
Moenomycins（Mm）是由Streptomyces ghanaensisATCC 14672产生的一种磷酸糖脂化合物，被认为是一类有前途的新型抗生素开发模型。在这方面，重要的是产生Mm过量生产菌株，这将是经济上合理生产该抗生素的基础。在这项工作中，一组基因的合成和接收的低分子量信号分子（LSM）在ATCC 14672中进行了描述和他们的意义Mm生产进行了研究。ATCC 14672基因组携带产生燕麦素和γ-丁内酯家族LSM的结构和调控基因。LSM生物合成基因sfg_07848和ssfg_07725的额外拷贝没有改变Mm生产水平。ATCC 14672 LSM不能恢复不产生丁内酯的S.灰色的同样，当异源宿主。lividans 1326产生Mm，其突变体M707（丁内酯合酶基因cbA缺陷）不产生Mm。因此，虽然ATCC 14672产生LSM的天然水平不限制Mm合成，但前者对于默诺霉素的合成是必不可少的。
Moenomycins (Mm)—phosphoglycolipid compounds produced byStreptomyces ghanaensisATCC14672—are considered a promising model for development of novel-class of antibiotics. In this regard it is important to generate Mm overproducing strains which would be a basis for economically justified production of this antibiotic. In this work a set of genes for synthesis and reception of low-molecular weight signaling molecules (LSM) in ATCC14672 were described and their significance for Mm production was studied. The ATCC14672 genome carries structural and regulatory genes for production of LSMs of avenolide and γ-butyrolactone families. Additional copies of LSM biosynthetic genesssfg_07848andssfg_07725did not alter the Mm production level. ATCC14672 LSMs are not capable of restoring the sporulation of butyrolactone-nonproducing mutant ofS. griseus. Likewise, while the heterologous hostS. lividans1326 produced Mm, its mutant M707 (deficient in the butyrolactone synthase genescbA) did not. Thus, while the natural level of LSMs production by ATCC14672 does not limit Mm synthesis, the former is essential for the synthesis of moenomycins.