Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)
Generation and analysis of 5318 expressed sequence tags from the filamentous sporophyte of Porphyra haitanensis (Rhodophyta)
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DOI：
10.1111/j.1529-8817.2007.00415.x
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发表时间：
2007-12-01
影响因子：
2.9
通讯作者：
Wang Guangce
中科院分区：
文献类型：
--
作者：
Fan Xiaolei;Fang Yongjun;Wang Guangce
坛紫菜T.J.Chang et B.F.Zeng(班加目，红藻门)产于中国，在亚洲广泛食用。为了更深入地了解其生理生化特性，我们从海棠的孢子体中获得了5318个表达序列标签(EST)，并将其组装成一个无冗余的集合，得到了2535个序列，其中只有32.2%(816)与已发表的序列(Nr和KOG)有一定的相似性。对这类EST的功能分类表明，大多数转录本与其保守的生物代谢有关，而海坦氏原虫很可能具有抗氰化物呼吸和类C4固碳途径，这两种途径以前从未在视紫藻类中报道过。28%的非冗余基因簇与条斑病菌孢子体中的基因有显著的相似性，其中16个上调基因在条斑病菌孢子体中也得到了大量表达。密码子使用分析表明，在海棠进化过程中可能存在高GC压力的暴露。这些发现代表了迄今为止最广泛的海棠EST集合，本研究中的所有EST都已提交给GenBank(登录号：DN604790-DN608469、EG016226-EG018540)。
Porphyra haitanensis T. J. Chang et B. F. Zheng (Bangiales, Rhodophyta) is cultivated in China and widely consumed in Asia. To gain more insight into its physiological and biochemical properties, we generated 5318 expressed sequence tags (ESTs) from the sporophyte of P. haitanensis, and upon assembling into a nonredundant set, 2535 sequences were obtained, among which only 32.2% (816) shared certain similarity with published sequences (Nr and KOG). Functional classification of such ESTs revealed that most of the transcripts were related to its conservative biological metabolism, and P. haitanensis most likely possesses cyanide-resistant respiration and a C4-like carbon-fixation pathway, both of which have never been reported in a rhodophyte before. Twenty-eight percent of the nonredundant gene clusters exhibited significant similarity to those from P. yezoensis Ueda sporophytes, and 16 genes up-regulated in P. yezoensis sporophytes were also expressed abundantly in P. haitanensis. Codon usage analysis indicated that exposure to high GC pressure might occur during evolution of P. haitanensis. These findings represent the most extensive collection of ESTs from P. haitanensis to date, and all the ESTs in this study have been submitted to GenBank (accession nos. DN604790-DN608469, EG016226-EG018540).