The serological prevalence and genetic diversity of hepatitis E virus in farmed rabbits in China
The serological prevalence and genetic diversity of hepatitis E virus in farmed rabbits in China
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DOI：
10.1016/j.meegid.2010.12.012
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发表时间：
2011-03-01
影响因子：
3.2
通讯作者：
Wang, Youchun
中科院分区：
文献类型：
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作者：
Geng, Yansheng;Zhao, Chenyan;Wang, Youchun
我们在中国养殖的獭兔中鉴定并鉴定了一种新型病毒，称为兔戊型肝炎病毒（HEV）。兔HEV在遗传上与其他已知的哺乳动物HEV和禽类HEV相关，但又不同，可能代表一种新的基因型。为了评估兔HEV的传播和遗传变异，在中国10个县共收集了1094份不同品种的兔血清样本。对所有血清进行抗HEV抗体、HEV抗原和病毒RNA的检测。来自10个县中的9个县的169份样本（15.4%）被发现HEV抗体阳性。血清阳性率以湖南省武汉市最高，为53.4%（55/103）。7个县检测到HEV抗原阳性，总患病率为3.7%（41/1094）。在22个样本中检测到HEV RNA，除1个样本外，其余样本均呈HEV抗原阳性。对开放阅读框2内304 bp扩增子的序列分析表明，本研究中所有的HEV分离株与已知的兔HEV毒株聚集在一个分支中，从基因型1到基因型4分开。兔HEV毒株具有遗传异质性，分为不同的类群。来自同一地理区域的菌株倾向于聚集在一起。这些结果表明，具有相当遗传多样性的兔hev在中国养殖家兔中普遍存在。兔戊型肝炎的潜在人畜共患风险需要进一步调查和评估。(C) 2010 Elsevier B.V.版权所有
We identified and characterized a novel virus, designated rabbit hepatitis E virus (HEV), in rex rabbits farmed in China. Rabbit HEV is genetically related to but distinct from other known mammalian HEVs and avian HEV and may represent a novel genotype. To evaluate the spread and genetic variation of rabbit HEV, a total of 1094 serum samples were collected from various breeds of rabbits across ten counties in China. All sera were screened for the presence of anti-HEV antibody, HEV antigen and viral RNA. A total of 169 samples (15.4%), from nine of the ten counties, were found to be positive for HEV antibody. The seroprevalence was highest in Wuhan, Hunan Province (53.4%, 55/103). Samples positive for HEV antigen were detected in seven counties and the overall prevalence was 3.7% (41/1094). HEV RNA was detected in 22 samples and all but one of these samples was found to be positive for HEV antigen. Sequence analysis of the 304 bp amplicons within open reading frame 2 showed that all HEV isolates in this study clustered with known rabbit HEV strains, in a branch separate from genotypes 1 to 4. The rabbit HEV strains were genetically heterogeneous and divided into divergent groups. Strains from the same geographic region tended to cluster together. These results indicate that rabbit HEVs with considerable genetic diversity are prevalent in farmed rabbits in China. The potential zoonotic risk of rabbit HEV needs to be investigated and evaluated further. (C) 2010 Elsevier B.V. All rights reserved.