Assessment of Candida albicans genes expressed during infections as a tool to understand pathogenesis.
Assessment of Candida albicans genes expressed during infections as a tool to understand pathogenesis.
复制标题
评估感染期间表达的白色念珠菌基因，作为了解发病机制的工具。
DOI：
10.1080/13693780410001722485
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发表时间：
2004
期刊：
影响因子：
2.9
通讯作者：
Clancy,CJ
中科院分区：
文献类型：
--
作者：
Nguyen,MHong;Cheng,S;Clancy,CJ
白色念珠菌是人类最常见的机会致病真菌，可引起皮肤粘膜、血液和深部器官感染。筛选C.在感染宿主中优先表达的白念珠菌基因代表了鉴定新的毒力因子和定义与发病机制相关的全局表达模式的策略。直到最近，C。白色念珠菌不适合使用现有技术进行筛选。随着新的分子遗传学工具的发展和C.白色念珠菌基因组在本文中，我们回顾了最近开发的技术，以确定基因表达的C。白色念珠菌感染期间，以及我们的实验室工作使用人类抗体为基础的战略。沿着其他人，我们已经证明，选择在体内表达的基因编码已知的和以前未认识的念珠菌毒力因子。未来在这一领域的研究将确定更多的新的毒力因子，以及推进我们对发病机制的理解。
Candida albicansis the most common fungal opportunistic pathogen of humans and causes mucocutaneous, bloodstream and deep organ infections. Screening forC. albicansgenes that are preferentially expressed within infected hosts represents a strategy to identify novel virulence factors and define global expression patterns relevant to pathogenesis. Until recently,C. albicanshas not been amenable to screening using existing technologies. This has begun to change with the development of new molecular genetic tools and the sequencing of theC. albicansgenome. In this paper, we review studies using recently developed techniques to identify genes expressed byC. albicansduring infections, as well as work from our laboratory using a human antibody-based strategy. Along with others, we have shown that selectedin vivoexpressed genes encode known and previously unrecognized candidal virulence factors. Future studies in this area will identify additional novel virulence factors, as well as advance our understanding of pathogenesis.