Enhancement of the antimicrobial properties of bacteriophage-K via stabilization using oil-in-water nano-emulsions.
Enhancement of the antimicrobial properties of bacteriophage-K via stabilization using oil-in-water nano-emulsions.
复制标题
通过使用水包油纳米乳液进行稳定化增强噬菌体-K 的抗菌特性。
DOI：
10.1002/btpr.1898
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发表时间：
2014
影响因子：
2.9
通讯作者：
Esteban PP
中科院分区：
文献类型：
--
作者：
Esteban PP
噬菌体疗法是一种很有前途的新疗法，可能有助于克服耐药病原菌构成的威胁，这些细菌在住院患者中越来越多地被发现。开发生物相容和可持续的载体，将活细菌病毒整合到伤口敷料中是一种有希望的替代方案。这篇文章评估了噬菌体K对金黄色葡萄球菌的抗菌效果，当它稳定下来并通过水包油纳米乳剂输送时。采用热相转化乳化法制备纳米乳状液，分别用天然乳状液、乳状液与K噬菌体复合乳状液刺激细菌生长，通过测定细菌样品的浊度来评价纳米乳状液对噬菌体的感染力，以及放置时间对细菌生长的影响。新制备的噬菌体K/纳米乳剂具有比自由悬浮噬菌体更强的抗菌活性。装载噬菌体的乳剂可导致三种不同菌株的金黄色葡萄球菌迅速而彻底的细菌死亡。在室温(18-20摄氏度)储存或在4摄氏度冷藏长达10天的制剂中也观察到了同样的效果。采用响应面试验设计，考察了乳剂对细菌生长和噬菌体裂解活性的相对影响。稀释后的乳状液对细菌生长的影响较小，稀释后的噬菌体乳剂具有较强的抗菌活性。通过纳米乳剂输送时，噬菌体活性的增强还没有报道。这促使人们进一步研究使用这些配方来开发新的抗微生物伤口管理策略。©2014美国化学工程师学会生物技术。程序，30：932-944,2014
Bacteriophage therapy is a promising new treatment that may help overcome the threat posed by antibiotic‐resistant pathogenic bacteria, which are increasingly identified in hospitalized patients. The development of biocompatible and sustainable vehicles for incorporation of viable bacterial viruses into a wound dressing is a promising alternative. This article evaluates the antimicrobial efficacy of Bacteriophage K against Staphylococcus aureus over time, when stabilized and delivered via an oil‐in‐water nano‐emulsion. Nano‐emulsions were formulated via thermal phase inversion emulsification, and then bacterial growth was challenged with either native emulsion, or emulsion combined with Bacteriophage K. Bacteriophage infectivity, and the influence of storage time of the preparation, were assessed by turbidity measurements of bacterial samples. Newly prepared Bacteriophage K/nano‐emulsion formulations have greater antimicrobial activity than freely suspended bacteriophage. The phage‐loaded emulsions caused rapid and complete bacterial death of three different strains of S. aureus. The same effect was observed for preparations that were either stored at room temperature (18–20°C), or chilled at 4°C, for up to 10 days of storage. A response surface design of experiments was used to gain insight on the relative effects of the emulsion formulation on bacterial growth and phage lytic activity. More diluted emulsions had a less significant effect on bacterial growth, and diluted bacteriophage‐emulsion preparations yielded greater antibacterial activity. The enhancement of bacteriophage activity when delivered via nano‐emulsions is yet to be reported. This prompts further investigation into the use of these formulations for the development of novel anti‐microbial wound management strategies. © 2014 American Institute of Chemical EngineersBiotechnol. Prog., 30:932–944, 2014