Synchronous inhibitory synaptic inputs to layer II/III pyramidal neurons in the murine barrel cortex
Synchronous inhibitory synaptic inputs to layer II/III pyramidal neurons in the murine barrel cortex
复制标题
小鼠桶状皮层 II/III 层锥体神经元的同步抑制性突触输入
DOI：
10.1016/j.brainres.2021.147686
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发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
Kobayashi Masayuki
中科院分区：
文献类型：
--
作者：
Yamamoto Kiyofumi;Nakaya Yuka;Sugawara Shiori;Kobayashi Masayuki
筒状皮层具有明显的柱状组织。虽然GABA能抑制在调节神经兴奋中起着至关重要的作用，以响应施加到胡须的机械刺激，在柱内和跨柱锥体神经元的抑制性突触传递的同步事件的配置文件仍然未知。为了探讨锥体神经元同步抑制的功能机制，我们进行了配对的全细胞膜片钳记录和记录自发抑制性突触后电流（sIPSC）从第II/III层锥体神经元。使用sIPSC的交叉相关图（Ims bin）来检测同步sIPSC。同步神经元对被定义为那些在-3到3 ms之间的sIPSC的峰值数量超过-50到50 ms期间的sIPSC数量减去-3到3 ms期间的数量的平均值+ 2 SD的神经元对。在位于同一列（柱内）的锥体神经元的记录中，61.5%的神经元对被归类为同步神经元对，而52.6%的锥体神经元对被定义为同步神经元对。在异步神经元对中，同步sIPSC的振幅与异步sIPSC的振幅相当，而在同步神经元对中，同步sIPSC的振幅大于异步sIPSC的振幅。sIPSC的同步性不依赖于神经元对的距离。这些结果表明，第II/III层锥体神经元接收由某种类型的GABA能中间神经元产生的同步抑制性突触输入，该中间神经元在锥体神经元中诱导大的IPSC，可能是快速尖峰细胞。
The barrel cortex exhibits obvious columnar organization. Although GABAergic inhibition plays a critical role in regulating neural excitation in response to mechanical stimuli applied to whiskers, the profiles of synchronous events for inhibitory synaptic transmission in intracolumnar and transcolumnar pyramidal neurons remain unknown. To explore a functional mechanism of synchronous inhibition of pyramidal neurons, we performed paired whole-cell patch-clamp recordings and recorded spontaneous inhibitory postsynaptic currents (sIPSCs) from layer II/III pyramidal neurons. A cross-correlogram of sIPSCs (1 ms bin) was used to detect synchronous sIPSCs. Synchronous neuron pairs were defined as those whose peak number of sIPSCs between −3 and 3 ms exceeded the mean + 2 SD of the number of sIPSCs in the period of −50 to 50 ms minus the number in that of −3 to 3 ms period. In the recording of pyramidal neurons located in the same column (intracolumn), 61.5% of neuron pairs were classified as synchronous neuron pairs, while 52.6% of pyramidal neuron pairs in adjacent columns (transcolumn) were defined as synchronous neuron pairs. The amplitude of synchronous sIPSCs was comparable to that of asynchronous sIPSCs in asynchronous neuron pairs, whereas that of synchronous sIPSCs was larger than that of asynchronous sIPSCs in synchronous neuron pairs. Synchronicity of sIPSCs did not depend on the distance of neuron pairs. These results suggest that layer II/III pyramidal neurons receive synchronous inhibitory synaptic inputs generated by a certain type of GABAergic interneuron that induces large IPSCs in pyramidal neurons, likely to be fast-spiking cells.