Phlorizin Prevents Electrically-Induced Ventricular Tachyarrhythmia during Ischemia in Langendorff-Perfused Guinea-Pig Hearts
Phlorizin Prevents Electrically-Induced Ventricular Tachyarrhythmia during Ischemia in Langendorff-Perfused Guinea-Pig Hearts
复制标题
DOI：
10.1248/bpb.b14-00046
复制
发表时间：
2014-07-01
影响因子：
2
通讯作者：
Yamada, Mitsuhiko
中科院分区：
文献类型：
--
作者：
Hirose, Masamichi;Shibazaki, Toshihide;Yamada, Mitsuhiko
连根茎苷是黄酮类化合物的一种，具有过氧亚硝酸盐清除作用。本研究旨在阐明苯连菌素对缺血性室性心动过速（VT）的作用。记录了42颗langendorff灌注豚鼠急性全脑缺血时心外膜表面光信号或左室舒张末期压（LVEDP）的变化。实验分别在对照条件下和过氧亚硝酸盐清除剂n -2-巯基丙基甘氨酸（2-MPG）存在下进行。三种干预措施在缺血20mm处的平均动作电位持续时间没有差异。在对照组中，脉冲传导在缺血20mm时随时间而减慢。在缺血15和20 mm时，苯连菌素而非2-MPG改善了缺血传导减慢。在对照组和2-MPG存在的情况下，程控刺激在20mm缺血时诱导室速，但在苯丙素存在的情况下不存在(p
Phlorizin is a type of flavonoids and has a peroxynitrite scavenging effect. This study aimed to elucidate the effects of phlorizin on ischemia-induced ventricular tachyarrhythmia (VT). Optical signals from the epicardial surface of the ventricle or left ventricular end diastolic pressure (LVEDP) were recorded during acute global ischemia in 42 Langendorff-perfused guinea pig hearts. Experiments were performed in the control condition and in the presence of phlorizin or N-2-mercaptopropionylglycine (2-MPG), a peroxynitrite scavenger, respectively. Mean action potential duration at 20 mm of ischemia did not differ among the three interventions. Impulse conduction time-dependently slowed during 20 mm of ischemia in the control. Phlorizin but not 2-MPG improved the ischemic conduction slowing at 15 and 20 mm of ischemia. Programmed stimulation induced VT at 20 mm of ischemia in the control and in the presence of 2-MPG but not in the presence of phlorizin (p