High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel
High-yield cell ordering and deterministic cell-in-droplet encapsulation using Dean flow in a curved microchannel
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DOI：
10.1039/c2lc00013j
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
van den Berg, Albert
中科院分区：
文献类型：
--
作者：
Kemna, Evelien W. M.;Schoeman, Rogier M.;van den Berg, Albert
在这篇文章中，我们描述了在一个简单弯曲的连续微通道中，利用单元的Dean耦合惯性有序，实现了单细胞液滴的高产量(77%)和高速(2700个细胞S(-1))的封装。通过引入Dean力，粒子在运动小于1 cm后将有序到一个平衡位置。我们在PDMS中使用了平面弯曲的微通道结构来对两种类型的髓系白血病细胞(HL60和K562细胞)进行空间排序，使得确定性的单细胞被包裹在微微升的液滴中。达到了高达77%的效率，克服了泊松统计对随机细胞加载施加的限制，后者只产生包含单个细胞的液滴的37%。此外，我们确认90%的细胞仍然存活。这种无源微流控方法提供的简单平面结构和高通量使其在基于液滴的平台上用于单电池应用的芯片实验室(LOC)设备中具有吸引力。
In this article high-yield (77%) and high-speed (2700 cells s(-1)) single cell droplet encapsulation is described using a Dean-coupled inertial ordering of cells in a simple curved continuous microchannel. By introducing the Dean force, the particles will order to one equilibrium position after travelling less than 1 cm. We use a planar curved microchannel structure in PDMS to spatially order two types of myeloid leukemic cells (HL60 and K562 cells), enabling deterministic single cell encapsulation in picolitre drops. An efficiency of up to 77% was reached, overcoming the limitations imposed by Poisson statistics for random cell loading, which yields only 37% of drops containing a single cell. Furthermore, we confirm that > 90% of the cells remain viable. The simple planar structure and high throughput provided by this passive microfluidic approach makes it attractive for implementation in lab on a chip (LOC) devices for single cell applications using droplet-based platforms.