Intervening transcribed spacer region 1 variability in Cyclospora cayetanensis.
Intervening transcribed spacer region 1 variability in Cyclospora cayetanensis.
复制标题
Cyclospora cayetanensis 中干预转录间隔区 1 的变异性。
DOI：
10.1128/jcm.38.6.2339-2343.2000
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发表时间：
2000
影响因子：
9.4
通讯作者：
Sterling,CR
中科院分区：
文献类型：
--
作者：
Adam,RD;Ortega,YR;Gilman,RH;Sterling,CR
卡耶坦环孢子虫是一种顶端复门原生动物寄生虫，已成为流行性和地方性腹泻的重要原因。水源性和食源性疫情都曾发生过，其中美国大量病例与从危地马拉进口的覆盆子有关。分子标记可用于追踪许多与水源性或食源性腹泻相关的细菌病原体的流行病学，例如血清分型和脉冲场电泳。然而，目前还没有针对 C 的分子标记。卡耶塔尼西斯。小亚基 rRNA 基因和大亚基 rRNA 基因之间的插入转录间隔区 (ITS) 区域表现出比小亚基 rRNA 序列本身更大的序列变异性，并且可用于其他生物体的分子分型。因此，ITS1 变异性可能允许识别 C 的不同基因型。卡耶塔尼西斯。为了确定 C 之间 ITS1 变异的程度。 Cayetanensisisolates，C的ITS1序列。我们从多种来源获得了卡耶坦尼西斯分离株，包括覆盆子相关病例、危地马拉病例以及秘鲁的汇总和个体分离株。所有 5 个覆盆子相关分离株的 ITS1 序列都是相同的，这与它们的单一来源一致。相比之下，两个危地马拉分离株之一和两个秘鲁分离株包含多个 ITS1 序列。这些多个序列可能代表来自单个临床来源的多个克隆，或更可能代表单个克隆基因组内 ITS1 区域的变异性。
Cyclospora cayetanensisis an apicomplexan protozoan parasite which has emerged as an important cause of epidemic and endemic diarrhea. Water-borne as well as food-borne outbreaks have occurred, including a large number of U.S. cases associated with raspberries imported from Guatemala. Molecular markers exist for tracing the epidemiology of many of the bacterial pathogens associated with water-borne or food-borne diarrhea, such as serotyping and pulsed-field electrophoresis. However, there are currently no molecular markers available forC. cayetanensis. The intervening transcribed spacer (ITS) regions between the small- and large-subunit rRNA genes demonstrate much greater sequence variability than the small-subunit rRNA sequence itself and have been useful for the molecular typing of other organisms. Thus, ITS1 variability might allow the identification of different genotypes ofC. cayetanensis. In order to determine the degree of ITS1 variability amongC. cayetanensisisolates, the ITS1 sequences ofC. cayetanensisisolates from a variety of sources, including raspberry-associated cases, cases from Guatemala, and pooled and individual isolates from Peru, were obtained. The ITS1 sequences of all five raspberry-associated isolates were identical, consistent with their origin from a single source. In contrast, one of the two Guatemala isolates and two Peruvian isolates contained multiple ITS1 sequences. These multiple sequences could represent multiple clones from a single clinical source or, more likely, variability of the ITS1 region within the genome of a single clone.