Improvement and development of rapid chromatographic strip-tests for the diagnosis of rinderpest and peste des petits ruminants viruses
Improvement and development of rapid chromatographic strip-tests for the diagnosis of rinderpest and peste des petits ruminants viruses
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DOI：
10.1016/j.jviromet.2011.03.016
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发表时间：
2011-06-01
影响因子：
3.1
通讯作者：
Anderson, John
中科院分区：
文献类型：
--
作者：
Bruening-Richardson, Anke;Akerblom, Lennart;Anderson, John
本文介绍了用于检测猪瘟病毒（RPV）的栏边快速诊断测试的改进，以及用于检测另一种麻疹病毒、小反刍兽疫病毒（PPRV）的类似测试的开发。使用 Svanova Biotech 格式，开发了用于 RPV 和 PPRV 检测的原型色谱条测试装置。对于 RP 装置，结合了单克隆抗体 (Mab)，可识别 RPV 谱系 2 的其他 RPV 毒株，增强了快速诊断测试的反应范围。该装置检测了实验性感染不同 RPV 毒株的动物体内的抗原。它还显示出与之前报道的 RP Clearview (TM) 设备类似的检测水平。此外，在巴基斯坦的现场条件下也检测到了 RPV。PPR 测试的开发使用了 PPRV 特异性单克隆抗体 (C77)。该 Mab 可识别多种 PPRV 分离株，并且与任何其他测试病毒没有表现出任何交叉反应。在动物实验中，该设备能够检测动物眼拭子中的病毒抗原。 PPRV 测试对于未来的 PPR 控制根除计划应该具有无价的价值。 (C) 2011 Elsevier B.V. 保留所有权利。
This paper describes the improvement of a rapid diagnostic test for the detection of rinderpest virus (RPV) at pen-side and the development of a similar test for the detection of another Morbillivirus, peste de petits ruminants virus (PPRV). Using the Svanova Biotech format, prototype chromatographic strip test devices were developed for RPV and PPRV detection. For the RP device, the incorporation of a monoclonal antibody (Mab), which recognises additional RPV strains of RPV lineage 2, enhanced the range of reactivity of the rapid diagnostic test. The device detected antigen in animals infected experimentally with different RPV strains. It also showed detection levels similar to the RP Clearview (TM) device reported previously. In addition, RPV was also detected under field conditions in Pakistan.A PPRV specific Mab (C77) was used for the development of the PPR test. This Mab recognised a wide range of PPRV isolates and did not show any cross-reactivity with any other virus tested. In animal experiments the device was able to detect viral antigen in eye swabs taken from the animals. The PPRV test should be invaluable for future PPR control eradication programs. (C) 2011 Elsevier B.V. All rights reserved.