SOMA NEUROFILAMENT IMMUNOREACTIVITY IS RELATED TO CELL-SIZE AND FIBER CONDUCTION-VELOCITY IN RAT PRIMARY SENSORY NEURONS
SOMA NEUROFILAMENT IMMUNOREACTIVITY IS RELATED TO CELL-SIZE AND FIBER CONDUCTION-VELOCITY IN RAT PRIMARY SENSORY NEURONS
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DOI：
10.1113/jphysiol.1991.sp018497
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发表时间：
1991-04-01
影响因子：
5.5
通讯作者：
WADDELL, PJ
中科院分区：
文献类型：
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作者：
LAWSON, SN;WADDELL, PJ
1. 细胞内记录在背根神经节在体外37 ℃。 使用46 - 51日龄雌性Wistar大鼠的L4、L5和L6神经节。 在每个神经元中测量传导速度（CV）并注射荧光染料。 随后，在注射染料的神经元中测量对RT97（磷酸化的200 kDa神经丝亚基的单克隆抗体）的免疫反应性的强度以及细胞大小（在核水平的横截面积）。 RT97用于区分L（亮，神经递质丰富）和SD（小，暗，神经递质贫乏）神经元胞体。 将神经元分类为C神经元（CV <1.3 m/s）、C/A-δ神经元（1.3 - 2 m/s）、A-δ神经元（2 - 12 m/s）或A-α/β神经元（> 12 m/s）。 所有A纤维胞体RT97阳性（L），所有C纤维胞体RT97阴性（SD），尽管在C/A-delta组中既看到阳性神经元也看到阴性神经元。 因此，RT97阴性体细胞具有C（无髓鞘）或C/A-delta纤维，而RT97阳性体细胞具有A（有髓鞘）或C/A-delta纤维。 A细胞和C细胞的大小分布分别与它们的L-和SD-细胞神经元的分类一致。 A-δ细胞的大小分布呈偏态，以小细胞为主，中等细胞次之. 细胞大小与纤维CV之间呈松散的正相关。 如果将所有神经元一起考虑，则RT97强度与CV正相关，但在C、A-delta或A-alpha/beta CV组内未观察到相关性。 当所有神经元一起考虑时，RT97强度与细胞大小呈正相关。 虽然在C或A-δ CV组中没有观察到相关性，但在A-α/β神经元中观察到明显的正相关性。 RT97强度与细胞大小的关系在对照实验中未被轴突切断、体外时间或细胞内染料的存在所明显改变。 新生大鼠神经节内可见RT97阴性和阳性神经元。 它们的大小分布分别类似于这个年龄段的SD和L神经元群体。 因此，RT97免疫反应性可能是一个有用的预测的细胞类型和有髓状态的感觉细胞是注定要达到成年大鼠。
1. Intracellular recordings were made in dorsal root ganglia in vitro at 37-degrees-C. The L4, L5 and L6 ganglia from 46- to 51-day-old female Wistar rats were used. In each neuron conduction velocity (CV) was measured and fluorescent dye was injected. Later the intensity of the immunoreactivity to RT97 (a monoclonal antibody to the phosphorylated 200 kDa neurofilament subunit) as well as the cell size (cross-sectional area at the nuclear level) were measured in the dye-injected neurons. RT97 was used to distinguish between the L (light, neurofilament-rich) and the SD (small dark, neurofilament-poor) neuronal somata.2. Neurons were classified as C neurons (CV < 1.3 m/s), C/A-delta neurons (1.3-2 m/s), A-delta neurons (2-12 m/s) or A-alpha/beta neurons (> 12 m/s).3. All A-fibre somata were RT97 positive (L) and all C-fibre somata were RT97 negative (SD), although in the C/A-delta group both positive and negative neurons were seen. Thus, RT97-negative somata had C (unmyelinated) or C/A-delta fibres, while RT97-positive somata had A (myelinated) or C/A-delta fibres.4. The size distributions of A neurons and C neurons were consistent with their classification as L- and SD-cell neurons respectively. The size distribution of A-delta cells was skewed with a peak of small cells and a tail of medium-size cells.5. There was a loose positive correlation between cell size and fibre CV.6. RT97 intensity was positively correlated with CV if all neurons were considered together, but no correlation was seen within the C, A-delta or A-alpha/beta CV groups.7. RT97 intensity was positively correlated with cell size when all neurons were considered together. Although no correlation was seen within the C or the A-delta CV groups, a clear positive correlation was seen for A-alpha/beta neurons.8. The relationship of RT97 intensity to cell size was not demonstrably altered by axotomy, time in vitro or the presence of intracellular dye in control experiments.9. RT97-negative and -positive neurons could be seen in neonatal rat ganglia. Their size distributions resembled, respectively, the SD- and L-neuron populations at this age. RT97 immunoreactivity may therefore be a useful predictor of the cell type and myelinated state which a sensory cell is destined to reach in the adult rat.