Simple method for the collection of pancreatic islets by the use of Ficoll-Conray gradient.
Simple method for the collection of pancreatic islets by the use of Ficoll-Conray gradient.
复制标题
使用 Ficoll-Conray 梯度收集胰岛的简单方法。
DOI：
10.1507/endocrj1954.26.495
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
S. Todo
中科院分区：
文献类型：
--
作者：
T. Okeda;J. Ono;R. Takaki;S. Todo
描述了从正常大鼠中收集大量活胰岛的简单方法。该方法涉及胶原酶消化胰腺，然后使用 Ficoll-Conray 不连续梯度将孤立的胰岛与不需要的腺泡碎片分离。 Ficoll-Conray 将未透析的 12.5% Ficoll 和 33.4% Conray 以 2:1 的比例混合，制备比重为 1.095、渗透压为 396 mOsm/l 的溶液。用蒸馏水稀释A溶液得到比重分别为1.084、1.072和1.048的溶液B、C和D。使用Ficoll-Conray梯度，从年轻成年大鼠胰腺中一致地收集了大约200个存活的胰岛，这些胰岛保持了良好的形态和功能。
Simple method for the collection of large numbers of viable pancreatic islets from normal rats is described. This method involves collagenase digestion of the pancreas, followed by the use of Ficoll-Conray discontinuous gradient for the separation of isolated islets from unwanted acinal debris. Ficoll-Conray A solution was prepared by mixing undialyzed 12.5% Ficoll and 33.4% Conray in the ratio 2 : 1, to make a specific gravity of 1.095 and osmolarity of 396 mOsm/l. The solution B, C, and D, with specific gravities 1.084, 1.072, and 1.048, respectively were obtained by diluting A solution with distilled water. Using Ficoll-Conray gradient, about 200 viable islets which maintained excellently their morphology and function, were collected consistently from a young adult rat pancreas.