Expression, exon-intron organization, and chromosome mapping of the human sodium iodide symporter
Expression, exon-intron organization, and chromosome mapping of the human sodium iodide symporter
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DOI：
10.1210/en.138.8.3555
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发表时间：
1997-08-01
期刊：
影响因子：
4.8
通讯作者：
Jhiang, SM
中科院分区：
文献类型：
--
作者：
Smanik, PA;Ryu, KY;Jhiang, SM
人甲状腺对碘的主动摄取是由人碘化钠同调体（hNIS）介导的。在本报告中，我们发现hNIS主要在甲状腺组织中表达，但也在乳腺、结肠和卵巢组织中表达。与正常甲状腺组织相比，hNIS在甲状腺肿瘤中的表达大大降低。在肿瘤组织中，hNIS的表达似乎是可变的，这与甲状腺癌对放射性碘治疗的可变反应一致。hNIS的编码区被14个内含子打断，并报道了每个外显子-内含子连接处的核苷酸序列。利用这些信息，确定了hNIS的另一种拼接形式。最后，hNIS基因的染色体定位在19p染色体上。
The active iodide uptake of the thyroid gland in humans is mediated by the human sodium iodide symporter (hNIS). In this report, we show that hNIS expression was detected primarily in thyroid tissue, but also in breast, colon and ovary tissues. Expression of hNIS is greatly reduced in thyroid tumors compared to normal thyroid tissue. Among tumor tissues, hNIS expression appears to be variable, consistent with the variable response to radioiodide treatment observed for thyroid carcinomas. The coding region of hNIS is interrupted by 14 introns, and the nucleotide sequence of each exon-intron junction is reported. Using this information, an alternatively spliced form of hNIS was identified. Finally, the chromosome location of the hNIS gene was mapped to chromosome 19p.