Analysis of specific IgM responses in secondary dengue virus infections: levels and positive rates in comparison with primary infections
Analysis of specific IgM responses in secondary dengue virus infections: levels and positive rates in comparison with primary infections
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DOI：
10.1016/j.jcv.2004.03.005
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发表时间：
2004-11-01
影响因子：
8.8
通讯作者：
Sawanpanyalert, P
中科院分区：
文献类型：
--
作者：
Chanama, S;Anantapreecha, S;Sawanpanyalert, P
背景：登革热病毒是世界热带和亚热带地区疾病的严重原因。实验室诊断对于确认登革热病毒感染至关重要。通过 IgM 捕获酶联免疫分析 (ELISA) 检测特异性 IgM 已被广泛用作主要的血清学诊断技术。目的：将继发性登革热病毒感染中的特异性 IgM 水平与原发感染中的特异性 IgM 水平进行比较。研究设计：通过 IgM 捕获 ELISA 检测了从 924 例确诊登革热病例中收集的总共 1780 个样本的抗登革热 IgM。结果与结论：所有原发性登革热病毒感染病例在发病第9天及以后均检测到特异性IgM。然而，28%（204/716）的继发感染病例中无法检测到特异性 IgM。原发感染的 IgM 平均滴度高于继发感染。结果证实IgM检测是原发性登革热病毒感染可靠的血清学诊断检测。虽然 IgM 检测也是一种有用的测试，但其他血清学诊断测试或登革热病毒检测测试对于确认所有继发性登革热病毒感染是必要的。 (C) 2004 Elsevier B.V. 保留所有权利。
Background: Dengue viruses are a serious cause of illness in tropical and subtropical areas of the world. Laboratory diagnosis is essential for confirmation of dengue virus infections. Detection of specific IgM by IgM-capture enzymed-linked immunoassay (ELISA) has been widely used as a main serological diagnostic technique. Objectives: The levels of specific IgM in secondary dengue virus infections were compared with those in primary infections. Study design: A total of 1780 samples collected from 924 confirmed dengue cases were tested for anti-dengue IgM by IgM-capture ELISA. Results and conclusions: Specific IgM was detected in all the cases with primary dengue virus infection on disease day 9 or later. However, specific IgM cannot be detected in 28% (204/716) of the cases in secondary infections. The average titers of IgM were higher in primary infections than in secondary infections. The results confirmed that IgM detection is a reliable serological diagnostic test in primary dengue virus infections. Although IgM detection is also a useful test, other serological diagnostic tests or tests for dengue virus detection are necessary for confirmation of all the secondary dengue virus infections. (C) 2004 Elsevier B.V. All rights reserved.