Epidemic spread of IncI1/pST113 plasmid carrying the Extended-Spectrum Beta-Lactamase (ESBL) blaCTX-M-8 gene in Escherichia coli of Brazilian cattle
Epidemic spread of IncI1/pST113 plasmid carrying the Extended-Spectrum Beta-Lactamase (ESBL) blaCTX-M-8 gene in Escherichia coli of Brazilian cattle
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DOI：
10.1016/j.vetmic.2020.108629
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发表时间：
2020-04-01
影响因子：
3.3
通讯作者：
Neto Ferreira, Helena Maria
中科院分区：
文献类型：
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作者：
Palmeira, Josman Dantas;Haenni, Marisa;Neto Ferreira, Helena Maria
导论：产广谱β -内酰胺酶（ESBL）的肠杆菌科的流行在世界范围内正在增加，农业食品部门是临床相关ESBL基因的储存库。我们的研究旨在检测和表征产生esbl的肠杆菌科，负责巴西牛的肠道定植。材料和方法：从巴西西北部健康牛的粪便样本中分离出产esbl的大肠杆菌。采用纸片扩散法测定药敏。通过PCR鉴定抗性和毒力基因，测序扩增子，PFGE和MLST鉴定克隆性，复制子分型、S1-PFGE和Southern blot杂交鉴定质粒。结合法检测ESBL基因的可转移性。结果：共鉴定出40株产esbl大肠杆菌，分别来自34/191只动物（17.8%）和15/22个养殖场（68.2%）。bla(CTX-M-)(8)基因是最常见的ESBL基因（62.5%），其次是bla(SHV-2a)（20.0%）、bla(CTX-M-)(2)（10.0%）和bla(CTX-M-)(15)（7.5%）。bla(CTX-M-)(8)基因在不相关动物和农场的多种大肠杆菌序列类型的IncI1/pST113质粒上定位。讨论：我们报告了巴西肉牛部门中产生esbl的大肠杆菌的首次表征和高流行率，这主要是由流行病IncI1/pST113/bla(CTX-M-)(8)质粒的传播支持的。由于巴西是世界上最大的牛肉出口国之一，因此应该注意到这种ESBL质粒在其他部门、国家和大陆的传播。
Introduction: The prevalence of Extended-Spectrum Beta-Lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide and the Agri-Food sector acts as a reservoir of clinically relevant ESBL genes. Our study aimed at detecting and characterizing ESBL-producing Enterobacteriaceae responsible for intestinal colonization of Brazilian bovines.Material and methods: ESBL-producing E. coli isolates were recovered from fecal samples of healthy cattle in Northwest Brazil. Antimicrobial susceptibility was determined by disk diffusion. Resistance and virulence genes were identified by PCR and amplicons were sequenced, clonality was assessed by PFGE and MLST, and plasmids were characterized by replicon typing, S1-PFGE and Southern blot hybridizations. Transferability of ESBL genes was assessed by conjugation assay.Results: A total of 40 ESBL-producing E. coli were characterized, which originated from 34/191 animals (17.8 %) and 15/22 farms (68.2 %). The bla(CTX-M-)(8) gene was the most frequent ESBL gene (62.5 %), followed by bla(SHV-2a) (20.0 %), bla(CTX-M-)(2) (10.0 %), and bla(CTX-M-)(15) (7.5 %). The bla(CTX-M-)(8) gene was localized on the IncI1/pST113 plasmid in multiple E. coli sequence types across unrelated animals and farms.Discussion: We report the first characterization and a high prevalence of ESBL-producing E. coli in the beef cattle sector in Brazil, which is mainly supported by the spread of an epidemic IncI1/pST113/bla(CTX-M-)(8) plasmid. Since Brazil is one of the biggest beef meat exporters worldwide, the spread of this ESBL plasmid across other sectors, countries and continents should be considered with attention.