Helicobacter pylori and two ultrastructurally distinct layers of gastric mucous cell mucins in the surface mucous gel layer
Helicobacter pylori and two ultrastructurally distinct layers of gastric mucous cell mucins in the surface mucous gel layer
复制标题
DOI：
10.1136/gut.49.4.474
复制
发表时间：
2001-10-01
期刊：
影响因子：
24.5
通讯作者：
Katsuyama, T
中科院分区：
文献类型：
--
作者：
Hidaka, E;Ota, H;Katsuyama, T
背景和目的：幽门螺杆菌不仅存在于胃粘膜表面粘液细胞的根尖表面，而且还存在于覆盖在胃粘膜的粘液凝胶层中。方法：对5例经Carnoy‘s液固定、石蜡包埋的人胃组织和10例经戊二醛固定的胃活检标本进行了光镜和电子显微镜观察。结果电子显微镜下，未见肠化的胃粘膜表层粘液细胞型粘蛋白和腺粘液型粘蛋白分别呈网状结构和带状结构。幽门螺杆菌多见于表面粘液细胞型粘蛋白的粘液凝胶层内的小聚集体中，这些聚集体中的幽门螺杆菌呈分裂状。幽门螺杆菌多见于与腺体粘液细胞粘蛋白交界处的表面粘液细胞型粘液凝胶层。结论：胃粘膜凝胶层中的两种胃粘蛋白在超微结构上存在差异。幽门螺杆菌优先在表面粘液细胞型粘蛋白的粘液凝胶层内定植并形成微集落。来自腺体粘液细胞的粘蛋白可能干扰幽门螺杆菌在粘液凝胶层内的运动。
Background and aims-Helicobacter pylori locate not only on the apical surface of surface mucous cells but also in the mucous gel layer covering the gastric mucosa. The present study was undertaken to observe the mucous gel layer itself and any H pylori in this layer at the electron microscopic level, and to determine whether H pylori proliferate in this layer.Methods-We examined resected human stomachs (five cases, fixed in Carnoy's solution, paraffin embedded) under the light microscope, and gastric biopsy specimens (10 cases, fixed in glutaraldehyde with or without osmium, epoxy embedded) under the electron microscope. We performed histochemical staining for gastric mucins and immunostaining for H pylori, gastric gland mucous type mucins, and intestinal mucins.Results-Under the electron microscope, surface mucous cell type mucins and gland mucous cell type mucins in the mucous gel layer covering gastric mucosa without intestinal metaplasia showed reticular and band like structures, respectively. H pylori were frequently found as small aggregates within the mucous gel layer of surface mucous cell type mucins, and H pylori within these aggregates were seen dividing. H pylori were frequently found in the mucous gel layer of the surface mucous cell type mucins along the border with the layer of gland mucous cell mucins. Occasionally, H pylori were trapped by frayed thin threads of the gland mucous cell type mucins.Conclusions-The two types of gastric mucins in the mucous gel layer differ in ultrastructure. H pylori preferentially colonise and form microcolonies within the mucous gel layer of surface mucous cell type mucins. Mucins from gland mucous cells may disturb the movement of H pylori within the mucous gel layer.