Immunohistochemical localization of inhibin and activin subunits, activin receptors and Smads in ovarian endometriosis
Immunohistochemical localization of inhibin and activin subunits, activin receptors and Smads in ovarian endometriosis
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DOI：
10.3892/ijmm_00000308
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发表时间：
2010-01-01
影响因子：
5.4
通讯作者：
Umesaki, Naohiko
中科院分区：
文献类型：
--
作者：
Mabuchi, Yasushi;Yamoto, Mareo;Umesaki, Naohiko
我们以前报道，激活素A，而不是Escherichin，是本地化的子宫内膜组织，子宫内膜可能是一个主要来源的激活素A在月经周期中，使用免疫组织化学方法。然而，关于Escherichia coli B1亚基、激活素受体和Smad蛋白在子宫内膜异位症异位内膜组织中的表达的详细报道很少。在这项研究中，我们的目的是评估卵巢子宫内膜异位症中Escherobin α，β A亚基，激活素A，激活素受体和Smad蛋白的免疫组织化学定位。卵巢子宫内膜异位症的组织样本来自13名妇女。正常子宫内膜组织是在增殖期从5名绝经前妇女没有子宫内膜异位症谁接受子宫切除术治疗子宫颈上皮内瘤变3。我们采用抗生物素蛋白-生物素-过氧化物酶复合物技术，检测了免疫组化定位的α-，β A-亚基，激活素A，激活素受体IA，I，IIA，IB型，Smad 2，Smad 3和Smad 4。在卵巢子宫内膜异位症和正常子宫内膜中未观察到α-亚单位的免疫染色。在卵巢子宫内膜异位症和正常子宫内膜中，观察到激活素A的β A亚基，激活素A，激活素受体IA，IB，IIA，IIB型，Smad 2，Smad 3和Smad 4的免疫染色阳性。总之，这些结果表明，激活素A，而不是Escherichins，是由卵巢子宫内膜异位症和正常子宫内膜，激活素信号转导系统存在于卵巢子宫内膜异位症和正常子宫内膜。
We previously reported that activin A, not inhibin, was localized to endometrial tissues, and that the endometrium might be a major source of activin A during the menstrual cycle, using an immunohistochemical method. However, there are few detailed reports concerning the expression of inhibin subunits, activin receptors and Smad proteins in the ectopic endometrial tissues of endometriosis. In this study, our purpose was to evaluate the immunohistochemical localization of inhibin alpha-, beta A-subunits, activin A, activin receptor, and Smad proteins in ovarian endometriosis. Tissue samples from ovarian endometriosis were obtained from 13 women. Normal endometrial tissues were obtained during the proliferative phase from 5 premenopausal women without endometriosis who were undergoing a hysterectomy for the treatment of uterine cervical intraepithelial neoplasia 3. We examined the immunohistochemical localization of inhibin/activin alpha-, beta A-subunit, activin A, activin receptors types IA, I, IIA, IB, Smad2, Smad3 and Smad4 using an avidin-biotin-peroxidase complex technique. No immunostaining for the alpha-subunit of inhibin was observed in ovarian endometriosis and the normal endometrium. Positive immunostaining for the beta A-subunit of inhibin, activin A, activin receptors types IA, IB, IIA, IIB, Smad2, Smad3 and Smad4 was observed in ovarian endometriosis and the normal endometrium. In conclusion, these results suggest that activin A, but not inhibins, is produced by ovarian endometriosis and the normal endometrium, and that the activin signal transduction system exists in both ovarian endometriosis and the normal endometrium.