Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection
Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection
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DOI：
10.1016/j.snb.2010.07.020
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发表时间：
2010-09-21
影响因子：
8.4
通讯作者：
Yun, Kwang-Seok
中科院分区：
文献类型：
--
作者：
Park, Hyunjin;Kim, Dongil;Yun, Kwang-Seok
本文介绍了一种微流控生物芯片的设计、制造和表征，该芯片具有集成的驱动电极和传感电极，驱动电极用于通过介电电泳操纵细胞和微珠，传感电极用于通过阻抗检测方法检测捕获。将偏转介电电泳屏障与受控压力驱动的液体流相结合允许精确控制悬浮液中的细胞/微珠。微珠捕获的阈值电压在不同的流速下进行了实验验证。测量了在捕获MCF 7细胞和聚苯乙烯微珠的情况下由感测电极之间的导电路径的阻塞引起的阻抗变化。在捕获MCF 7细胞之前，在1 kHz下的阻抗为10 - 9 M Ω，并且当将细胞放置在感测电极之间时增加到12 M Ω。(C)2010爱思唯尔B V版权所有
This paper presents the design, fabrication, and characterization of a microfluidic biochip with integrated actuation electrodes used to manipulate a cell and a microbead by dielectrophoresis and sensing electrodes to detect the trapping by using the impedance detection method. Combining deflective dielectrophoretic barriers with controlled pressure-driven liquid flows allows the accurate control of a cell/microbead in suspensions. The threshold voltage for microbead trapping was experimentally verified at various flow rates. The impedance change caused by the blockage of the electrical conducting path between sensing electrodes with the trapping of an MCF7 cell and a polystyrene microbead was measured The impedance before the trapping of an MCF7 cell was 10 9 M Omega at 1 kHz and increased to 12 M Omega when the cell was placed between sensing electrodes. (C) 2010 Elsevier B V All rights reserved