Expression of interleukin-6 in human craniopharyngiomas: a possible inducer of tumor-associated inflammation.
Expression of interleukin-6 in human craniopharyngiomas: a possible inducer of tumor-associated inflammation.
复制标题
人颅咽管瘤中白细胞介素 6 的表达：肿瘤相关炎症的可能诱导剂。
DOI：
10.3892/ijmm.14.4.505
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发表时间：
2004
影响因子：
5.4
通讯作者：
J. Kuratsu
中科院分区：
文献类型：
--
作者：
Masanao Mori;H. Takeshima;J. Kuratsu
颅咽管瘤的完全切除可能是困难的，因为它们倾向于粘附到周围的重要结构。通常，邻近的脑组织被白细胞浸润，并且由肿瘤细胞产生的细胞因子可能在组织反应中起关键作用。为了阐明这种局部炎症的分子机制，我们采用RT-PCR、免疫印迹和免疫组织化学相结合的方法研究了15例颅咽管瘤中白细胞介素（IL）-1 α、白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α的表达。尽管RT-PCR检测到IL-1 α、IL-6和TNF-α的表达，但免疫印迹显示仅IL-6有显著的蛋白表达。免疫组织化学研究发现，上皮细胞层是强免疫反应的IL-6。肿瘤细胞层对IL-1 α和TNF-α染色较弱或呈阴性。双抗体夹心ELISA法检测结果显示，囊肿液中IL-1 α和TNF-α水平显著高于脑脊液（CSF），但低于10倍。由于囊液中IL-6的浓度是脑脊液中IL-6浓度的50,000倍以上，我们认为IL-6在颅咽管瘤与脑实质交界面的炎症反应中起重要作用。
The total removal of craniopharyngiomas can be difficult because of their tendency to adhere to surrounding vital structures. Frequently, adjacent brain tissue is infiltrated by leukocytes and cytokine(s) produced by tumor cells may play a pivotal role in tissue reaction. To elucidate the molecular mechanism(s) underlying such local inflammation, we investigated the expression of interleukin (IL)-1alpha, interleukin (IL)-6, and tumor necrosis factor (TNF)-alpha in 15 craniopharyngiomas using a combination of RT-PCR, immunoblots, and immunohistochemistry. Although RT-PCR detected the expression of IL-1alpha, IL-6, and TNF-alpha, immunoblots demonstrated remarkable protein expression only for IL-6. Immunohistochemical study found that the epithelial cell layer was strongly immunoreactive for IL-6. Tumor cell layers stained weakly or negatively for IL-1alpha and TNF-alpha. Sandwich ELISA showed that levels of IL-1alpha and TNF-alpha were significantly higher in the cyst fluid than in cerebrospinal fluid (CSF) but lower than 10-fold. As the concentration of IL-6 in cyst fluid was >50,000 times that in CSF, we suggest that IL-6 plays an important role in the inflammatory reaction that occurs in the interface between the craniopharyngioma and the brain parenchyma.