Identification of secondary metabolites from Streptomyces violaceoruber TU22 by means of on-flow LC-NMR and LC-DAD-MS
Identification of secondary metabolites from Streptomyces violaceoruber TU22 by means of on-flow LC-NMR and LC-DAD-MS
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DOI：
10.1002/mrc.1633
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发表时间：
2005-09-01
影响因子：
2
通讯作者：
Mügge, C
中科院分区：
文献类型：
--
作者：
Pham, LH;Vater, J;Mügge, C
为了快速筛选放线菌的天然产物，LC-NMR， LC-DAD-MS和HPLC-PDA的在线耦合组合以及MALDI-TOF-MS特别合适。同时使用这些偶联技术为快速鉴定混合物中的天然化合物提供了相当大的优势。我们对紫色链霉菌TU 22次生代谢产物的研究结果表明，鉴定出的代谢产物中有50%以上是新化合物。利用LC-NMR、LC-MS/MS和-MSn技术结合二维核磁共振波谱技术，对4个新聚酮（granaticin C、metenaticin A、B和C）和4个已知聚酮（granaticin A、granatomycin E、daidzein和genistein）的结构进行了鉴定。(c)版权所有2005 John Wiley & Sons， Ltd。
For rapid screening of natural products from Actinomycetes, a combination of on-line couplings LC-NMR, LC-DAD-MS and HPLC-PDA, as well as MALDI-TOF-MS is particularly suitable. Simultaneous use of these coupling techniques provides considerable advantages for the rapid identification of natural compounds in mixtures.The results of our present investigation on secondary metabolite products of Streptomyces violaceoruber TU 22 showed that more than 50% of the identified metabolites are new compounds. The structures of four new polyketides (granaticin C, metenaticin A, B and C) as well as four known ones (granaticin A, granatomycin E, daidzein and genistein) have been elucidated using LC-NMR, LC-MS/MS and -MSn techniques in combination with two-dimensional NMR spectroscopy. (c) Copyright 2005 John Wiley & Sons, Ltd.