Carboxylic acid reductase: Structure and mechanism
Carboxylic acid reductase: Structure and mechanism
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DOI：
10.1016/j.jbiotec.2019.10.010
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发表时间：
2020-01-10
影响因子：
4.1
通讯作者：
Leys, David
中科院分区：
文献类型：
--
作者：
Gahloth, Deepankar;Aleku, Godwin A.;Leys, David
羧酸还原酶（CAR）是一种多结构域的大蛋白，催化多种酸类物质依赖ATP和NADPH还原为相应的醛类。这种特殊的反应具有相当大的生物技术意义。分离结构域、双结构域和全长CAR酶的结构和溶液研究的最新进展揭示了对羧酸还原活性机制的有价值的见解。本文综述了CAR的系统发育、序列和结构，以及这些观察结果的影响，以提高羧酸还原活性，将CAR开发为强大的生物催化剂。
Carboxylic acid reductase (CAR) enzymes are large multi-domain proteins that catalyse the ATP- and NADPH-dependent reduction of wide range of acids to the corresponding aldehydes. This particular reaction is of considerable biotechnological interest. Recent advances in the structural and solution studies of isolated domain, di-domain and full-length CAR enzymes revealed valuable insights into the mechanism of carboxylic acid reduction activity. This review features the phylogenetic, sequence and structural insight into the CAR and implications of these observations in order to improve carboxylic acid reduction activity to develop CAR as robust biocatalyst.