Pentazocine monitoring in rat hair and plasma by HPLC-fluorescence detection with DIB-Cl as a labelling reagent.
Pentazocine monitoring in rat hair and plasma by HPLC-fluorescence detection with DIB-Cl as a labelling reagent.
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使用 DIB-Cl 作为标记试剂，通过 HPLC 荧光检测来监测大鼠毛发和血浆中的喷他佐辛。
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
K. Nakashima
中科院分区：
文献类型：
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作者：
M. Wada;Rie Kurogi;A. Kaddoumi;M. Nakashima;K. Nakashima
采用 4-(4,5-二苯基-1H-咪唑-2-基)苯甲酰氯 (DIB-Cl) 作为标记试剂，环佐辛 (CZ) 作为内标 (IS)，通过 HPLC 荧光检测法测定大鼠毛发和血浆中的喷他佐辛 (PZ)。从头发或血浆样品中提取的PZ和IS用DIB-Cl衍生化，并用固相萃取净化所得溶液。通过 Wakopak Handy-ODS 柱（250 x 4.6 mm i.d.），使用由 0.1 mol/L 乙酸盐缓冲液（pH 6.2）：乙腈（25:75，v/v）组成的流动相，可以在 20 分钟内实现 DIB-PZ 和 -CZ 的等度分离。信噪比为 3 时，PZ 对大鼠毛发和血浆的检测限分别为 0.18 ng/mg 和 0.57 ng/mL。通过 IS 方法可获得可重复且精确的结果，日内和日间测量的 RSD 值小于 6.6%。该方法成功应用于单次给予 25 mg/kg PZ 后 Zucker 大鼠毛发和血浆样品中 PZ 水平的监测。此外，还评估了PZ掺入Zucker大鼠的黑色和白色毛发中的比率。
Pentazocine (PZ) in rat hair and plasma was determined by HPLC-fluorescence detection with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a labelling reagent and cyclazocine (CZ) as an internal standard (IS). PZ and IS extracted from hair or plasma sample were derivatized with DIB-Cl and the resulted solution was cleaned up with solid phase extraction. The isocratic separation of DIB-PZ and -CZ within 20 min could be achieved by a Wakopak Handy-ODS column (250 x 4.6 mm i.d.) using a mobile phase composed of 0.1 mol/L acetate buffer (pH 6.2):acetonitrile (25:75, v/v). The detection limits of PZ at a signal-to-noise ratio of 3 for rat hair and plasma were 0.18 ng/mg and 0.57 ng/mL, respectively. Reproducible and precise results could be obtained by an IS method with RSD values less than 6.6% for within- and between-day measurements. The method was successfully applied for the monitoring of PZ levels in Zucker rat hair and plasma samples after a single administration of 25 mg/kg PZ. Moreover, incorporation rates of PZ into black and white hair of Zucker rat were evaluated.
影响因子：
2.5
作者：
Rollins, DE;Wilkins, DG;Slawson, MH
通讯作者：
Slawson, MH