PANCREATIC-ISLETS - A TISSUE RICH IN SEROTONIN
PANCREATIC-ISLETS - A TISSUE RICH IN SEROTONIN
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DOI：
10.2337/diab.29.4.304
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发表时间：
1980-01-01
期刊：
影响因子：
7.7
通讯作者：
FELDMAN, JM
中科院分区：
文献类型：
--
作者：
BIRD, JL;WRIGHT, EE;FELDMAN, JM
尽管外源性5 -羟色胺[S]在多种物种中都是胰岛素分泌的抑制剂，但许多研究者怀疑内源性5 -羟色胺是否在调节胰岛素分泌中起作用。这是因为，除豚鼠外，大多数动物的胰岛没有S的组织化学证据。将胶原酶分离的金仓鼠胰岛中S的含量与其他已知含S的仓鼠组织中S的含量进行比较。后者通过羟吲哚- o -甲基转移酶和氚化s -腺苷蛋氨酸转化为褪黑激素。在对照仓鼠中，平均值是+-。平均浓度的标准误差，单位为。Mol /kg胰岛湿wt(26.3。9.00)大于胰腺（4.0 .+-）。0.84)，垂体前叶（6.3 .+-）。1.53)，中隆起(2.5 .+-。0.69)和大脑皮层(1.9 .+-。0.30)。单胺氧化酶抑制剂丙氨酰环丙胺后，组织S无明显升高。单独给鼠S前体5-羟色氨酸时，胰岛、胰腺和垂体前叶S分别增加22倍、31倍和5倍。在给药后再给5-羟色氨酸，胰岛、胰腺、垂体前叶和正中嵴S分别增加58倍、73倍、14倍和10倍。给药利血平后组织S降低，给药对氯苯丙氨酸后组织S降低。暴露于胶原酶不改变对照或药物治疗的仓鼠胰腺或大脑皮层的S浓度。仓鼠接受三酰环丙胺加5-羟色氨酸后，其血浆葡萄糖浓度升高（266 .+-）。19 vs. 116。8 mg/dl)，血浆胰岛素浓度降低(19.6 +-。3 . vs. 46 .+-8 .mu。U/ml)，血浆胰岛素/葡萄糖比(0.06 .+-。0.01 vs. 0.40。0.06)比对照仓鼠高。经化学分析，没有S组织化学证据的动物胰岛可能有较高的S浓度；胰岛S浓度的大量增加可能抑制了胰腺胰岛素的分泌。
Despite the fact that exogenous serotonin [S] is an inhibitor of insulin secretion in a variety of species, many investigators doubt that endogenous S plays a role in the regulation of insulin secretion. This is because, with the exception of the guinea pig, most animals do not demonstrate histochemical evidence of S in their pancreatic islets. The S content of collagenase-isolated golden hamster islets was compared with the S content of other hamster tissues known to contain S. In the S assay, S was converted to N-acetyl serotonin by N-acetyltransferase; the latter is converted to tritiated melatonin by hydroxyindole-O-methyltransferase and tritiated S-adenosylmethionine. In control hamsters, the mean .+-. standard error of the mean concentration in .mu.mol/kg wet wt of islets (26.3 .+-. 9.00) was greater than that of pancreas (4.0 .+-. 0.84), anterior pituitary (6.3 .+-. 1.53), median eminence (2.5 .+-. 0.69) and cerebral cortex (1.9 .+-. 0.30). There was no significant increase in tissue S after the administration of the monoamine oxidase inhibitor tranylcypromine. When the S precursor 5-hydroxytryptophan alone was given to hamsters, there was a 22-, 31- and 5-fold increase in islet, pancreatic and anterior pituitary S, respectively. When 5-hydroxytryptophan was given to hamsters after tranylcypromine administration, there was a 58-, 73-, 14- and 10-fold increase in islet, pancreatic, anterior pituitary and median eminence S, respectively. Tissue S decreased after administration of reserpine but not after administration of p-chloro-phenylalanine. Exposure to collagenase did not alter the S concentration of pancreas or cerebral cortex in control or drug-treated hamsters. Hamsters receiving tranylcypromine plus 5-hydroxytryptophan had a higher plasma glucose concentration (266 .+-. 19 vs. 116 .+-. 8 mg/dl), lower plasma insulin concentration (19 .+-. 3 vs. 46 .+-. 8 .mu.U/ml) and lower plasma insulin/glucose ratio (0.06 .+-. 0.01 vs. 0.40 .+-. 0.06) than control hamsters. Animals whose islets do not have histochemical evidence of S may have a high S concentration by chemical analysis; large increases in islet S concentration probably inhibit pancreatic insulin secretion.