The production of distinct forms of plasminogen activator by mouse embryonic cells.
The production of distinct forms of plasminogen activator by mouse embryonic cells.
复制标题
小鼠胚胎细胞产生不同形式的纤溶酶原激活剂。
DOI：
10.1016/0012-1606(82)90220-2
复制
发表时间：
1982
影响因子：
2.7
通讯作者：
S. Strickland
中科院分区：
文献类型：
--
作者：
K. Marotti;D. Belin;S. Strickland
我们检测了培养的小鼠胚胎的顶叶内胚层、内脏内胚层和胚外中胚层细胞产生蛋白酶纤溶酶原激活剂的情况。所有这些细胞类型都能合成和分泌这种酶，但纤溶酶原激活剂的分子特征不同，这取决于十二烷基硫酸钠-聚丙烯酰胺凝胶的表观分子量，两种抗血清对不同的纤溶酶原激活剂的抗原特性，以及与胎牛血清中存在的抑制剂的相互作用。顶叶内胚层纤溶酶原激活剂的主要分子量为79,000，免疫沉淀并被针对人类黑色素瘤纤溶酶原激活剂的抗血清抑制，但不被针对小鼠尿激酶的抗血清抑制，并且仅被血清抑制剂部分抑制。内脏内胚层和胚胎外中胚层纤溶酶原激活剂在所有标准上都是相同的，分子量为48,000，仅被抗尿激酶抗体灭活，并被胎牛血清中的抑制剂抑制。这些结果证实在早期小鼠胚胎中至少存在两种不同形式的纤溶酶原激活物。由顶叶内胚层产生的酶的独特性质可以作为这种细胞类型在这个发育阶段的诊断标记。当F9畸胎瘤干细胞被维甲酸和二丁基cAMP诱导分化时，它们分泌一种壁内胚层型纤溶酶原激活剂。
We have examined cultured parietal endoderm, visceral endoderm, and extraembryonic mesoderm cells from the mouse embryo for production of the protease plasminogen activator. All of these cell types synthesize and secrete the enzyme, but the molecular characteristics of the plasminogen activators differ as defined by the apparent molecular weight in sodium dodecyl sulfate-polyacrylamide gels, the antigenic properties as defined by two antisera to distinct plasminogen activators, and the interaction with an inhibitor present in fetal bovine serum. The parietal endoderm plasminogen activator has a predominant molecular weight of 79,000, is immunoprecipitated and inhibited by an antiserum raised against a human melanoma plasminogen activator, but not by an antiserum against mouse urokinase, and is only partially inhibited by the serum inhibitor. The visceral endoderm and extraembryonic mesoderm plasminogen activators, which are identical by all criteria, have molecular weights of 48,000, are inactivated only by the anti-urokinase antibodies, and are inhibited by an inhibitor in fetal bovine serum. These results establish the presence of at least two different forms of plasminogen activator in the early mouse embryo. The distinctive nature of the enzyme produced by parietal endoderm can be used as a diagnostic marker for this cell type at this stage of development. When F9 teratocarcinoma stem cells are induced to differentiate by retinoic acid and dibutyryl cAMP, they secrete a plasminogen activator of the parietal endoderm type.