The Regional Specificity of Mucosa-Associated Microbiota in Patients with Distal Colorectal Cancer
The Regional Specificity of Mucosa-Associated Microbiota in Patients with Distal Colorectal Cancer
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远端结直肠癌患者粘膜相关微生物群的区域特异性
DOI：
10.1159/000519487
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发表时间：
2022
期刊：
影响因子：
3.2
通讯作者：
Horie Hisanaga
中科院分区：
文献类型：
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作者：
Kono Yoshihiko;Inoue Ryo;Teratani Takumi;Tojo Mineyuki;Kumagai Yuko;Morishima So;Koinuma Koji;Lefor Alan Kawarai;Kitayama Joji;Sata Naohiro;Horie Hisanaga
背景/目的最近的研究表明，在结直肠癌（CRC）患者的粪便样本中，几种微生物的数量显著增加，表明它们参与了CRC的发展。本研究的目的是确定远端crc中增加的微生物，并确定肿瘤周围粘膜组织中增加的微生物的具体位置。方法收集手术切除的28例远端crc组织标本。每个标本取5份标本（位置A：肿瘤，B：邻近正常粘膜，C：肿瘤近1 cm处正常粘膜，D：近3 cm处正常粘膜，E：近6 cm处正常粘膜）。采用16S rRNA基因扩增子测序对样品中的微生物群进行分析，并比较5个地点微生物群的相对丰度（RA）。结果在属水平上，5个点中，A点梭杆菌和链球菌的RA最高，与e点差异显著，各属优势种为核梭杆菌和血管链球菌。这些物种的RAs值从B点到E点逐渐降低，在具核褐飞虱中差异有统计学意义。Peptostreptococcus属也表现出类似的趋势，a位Peptostreptococcus stomatis的RA与肿瘤浸润深度和肿瘤大小显著相关。结论尽管临床相关性尚不明确，但这些结果表明核梭菌、血管梭菌和口梭菌可以扩散到邻近的正常组织，并可能改变周围的微环境，支持结直肠癌的进展。
Background/AimsRecent studies have demonstrated that the populations of several microbes are significantly increased in fecal samples from patients with colorectal cancer (CRC), suggesting their involvement in the development of CRC. The aim of this study was to identify microbes which are increased in distal CRCs and to identify the specific location of microbes increased in mucosal tissue around the tumor.MethodsTissue specimens were collected from surgical resections of 28 distal CRCs. Five samples were collected from each specimen (location A: tumor, B: adjacent normal mucosa, C: normal mucosa 1 cm proximal to the tumor, D: normal mucosa 3 cm proximally, and E: normal mucosa 6 cm proximally). The microbiota in the sample were analyzed using 16S rRNA gene amplicon sequencing and the relative abundance (RA) of microbiota compared among the 5 locations.ResultsAt the genus level, the RA of Fusobacterium and Streptococcus at location A was the highest among the 5 locations, significantly different from that in location E. The dominant species of each genus was Fusobacterium nucleatum and Streptococcus anginosus. The RAs of these species gradually decreased from locations B to E with a statistically significant difference in F. nucleatum. The genus Peptostreptococcus also showed a similar trend, and the RA of Peptostreptococcus stomatis in location A was significantly associated with depth of tumor invasion and tumor size.ConclusionAlthough the clinical relevance is not clear yet, these results suggest that F. nucleatum, S. anginosus, and P. stomatis can spread to the adjacent normal tissues and may change the surrounding microenvironment to support the progression of CRC.