Conservation of the 17-kilodalton antigen gene within the genus Bartonella.
Conservation of the 17-kilodalton antigen gene within the genus Bartonella.
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巴尔通体属内 17 千道尔顿抗原基因的保守性。
DOI：
10.1128/cdli.7.2.251-257.2000
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Anderson,B
中科院分区：
文献类型：
--
作者：
Sweger,D;Resto-Ruiz,S;Johnson,DP;Schmiederer,M;Hawke,N;Anderson,B
The 17-kDa antigen ofBartonella henselaehas previously been shown to elicit a strong humoral immune response in patients with cat scratch disease (CSD) and to be useful in screening human serum samples for CSD. In this study, PCR amplification of genes homologous to the 17-kDa antigen gene ofB. henselaewas performed using genomic DNAs from several species ofBartonella, including the currently recognized human pathogens. Amplicons of similar size were demonstrated using the following chromosomal DNA templates:B. henselae(two strains),B. quintana(two strains),B. elizabethae,B. clarridgeiae,B. vinsoniisubsp.vinsonii, andB. vinsoniisubsp.berkhoffii. No evidence of aB. bacilliformishomolog of the 17-kDa antigen gene was obtained using multiple primer pairs. DNA sequencing revealed open reading frames capable of coding for proteins with sizes similar to that of the 17-kDa antigen ofB. henselaein all of the amplicons; however, extensive sequence divergence across the genus was noted. Cloning of the amplified products into pUC19 resulted in recombinants that directed synthesis of homologs of the 17-kDa protein. Immunoblot analysis using human sera from CSD cases demonstrated very little cross-reactivity among different species for this protein. In contrast, immunoblots using rabbit serum raised to the recombinantB. henselaeantigen showed extensive cross-reactivity with the proteins of otherBartonellaspecies. The data suggest that the use of the 17-kDa antigen as a serologic reagent may allow the development of more specific diagnostic assays. Furthermore, the nucleotide sequences from the various versions of the 17-kDa antigen gene should be useful for rapid identification ofBartonellaat the species level.
