Multifunction of the ER P-Type Calcium Pump Spf1 During Hyphal Development in Candida albicans
Multifunction of the ER P-Type Calcium Pump Spf1 During Hyphal Development in Candida albicans
复制标题
ER P 型钙泵 Spf1 在白色念珠菌菌丝发育过程中的多功能
DOI：
10.1007/s11046-019-00372-5
复制
发表时间：
2019
期刊：
影响因子：
5.5
通讯作者：
Mingchun Li
中科院分区：
文献类型：
--
作者：
Qilin Yu;Tianyu Ma;Congcong Ma;Biao Zhang;Mingchun Li
Candida albicans is one of the most important fungal pathogens. Hyphal development is required for the virulence of this pathogen. Our previous study has revealed that Spf1, an ER P-type calcium pump, plays an important role in hyphal development. However, the detailed mechanisms by which this protein functions in this process remain to be investigated. In this study, we found that loss of Spf1 led to decreased growth biomass under the hypha-inducing condition, suggesting a role of this protein in maintaining hyphal growth rate. Actin staining further revealed that the spf1Δ/Δ mutant showed attenuated tip-localization of actin patches and the defect in transport of both the chitin synthase Chs3 and the hypha-related factor Hwp1, implying that Spf1 functions in polarized growth of the hyphae by regulating actin organization and consequent polarized transport of morphogenesis-associated factors. Moreover, deletion of SPF1 led to abnormal vacuolar morphology under the hypha-inducing condition, which may also contribute to the defect of hyphal development in the spf1Δ/Δ mutant. This study revealed the pleiotropic role of Spf1-regulated calcium homeostasis in controlling hyphal development in C. albicans.
