Oral glutamine reduces bacterial translocation following abdominal radiation.
Oral glutamine reduces bacterial translocation following abdominal radiation.
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DOI：
10.1016/0022-4804(90)90136-p
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
W. Souba;V. Klimberg;R. Hautamaki;William H. Mendenhall;F. Bova;R. Howard;K. Bland;E. Copeland
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文献类型：
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作者：
W. Souba;V. Klimberg;R. Hautamaki;William H. Mendenhall;F. Bova;R. Howard;K. Bland;E. Copeland
在大鼠接受单剂量腹部放射（1000拉德）后，研究了膳食谷氨酰胺对细菌易位的影响，这种放射会导致可重复的粘膜损伤，并导致放射后（XRT）肠系膜淋巴结培养阳性的高发生率。 XRT 后，大鼠仅在饮用水中摄入氨基酸谷氨酰胺（3%，+GLN）或对照非必需氨基酸（甘氨酸，-GLN）。饮食是等氮和等容的。 XRT 后四天，将大鼠麻醉并进行剖腹手术。无菌切除并培养肠系膜淋巴结。还获取动脉血用于全血谷氨酰胺测定。对照大鼠未接受 XRT，但接受相同的饮食。在接受不含 GLN 饮食的 XRT 大鼠中，肠系膜淋巴结培养阳性的发生率为 89%（9 只大鼠中的 8 只），而在接受富含 GLN 饮食的放射大鼠中，发生率降至 20%（P<0.05）。在接受富含 GLN 和缺乏 GLN 饮食 4 天的非辐射对照大鼠中，分别有 8 只大鼠中的 0 只和 8 只大鼠中的 1 只发生细菌易位 (NS)。向 XRT 大鼠提供谷氨酰胺会导致血液中谷氨酰胺水平升高（408 ± 25 μMin XRT +GLN 对比 311 ± 19 μMin XRT -GLN，P< 0.05）。此外，提供 GLN 可以维持粘膜质量并减少体重减轻（P < 0.05）。这些数据进一步支持了这样的假设：谷氨酰胺有助于维持肠粘膜屏障，从而降低肠道损伤后细菌易位的发生率。讨论了谷氨酰胺降低腹部 XRT 后肠系膜淋巴结培养阳性发生率的可能机制。
The effect of dietary glutamine on bacterial translocation was studied in rats following administration of a single dose of abdominal radiation (1000 rad) that causes a reproducible mucosal injury and results in a high incidence of culture-positive mesenteric lymph nodes after radiation (XRT). Following XRT, rats received only the amino acid glutamine (3%, +GLN) in their drinking water or a control nonessential amino acid (glycine, −GLN). Diets were isonitrogenous and isovolumetric. Four days after XRT, rats were anesthetized and a laparotomy was performed. Mesenteric lymph nodes were sterilely excised and cultured. Arterial blood was also obtained for whole blood glutamine determination. Control rats received no XRT but received identical diets. In XRT rats who received the GLN-free diet, the incidence of culture positive mesenteric lymph nodes was 89% (eight of nine rats) while in the radiated rats receiving the GLN-enriched diet, the incidence fell to 20% (P< 0.05). In non-radiated control rats receiving GLN-enriched and GLN-depleted diets for 4 days, bacterial translocation occurred in zero of eight and one of eight rats, respectively (NS). Provision of glutamine to XRT rats resulted in higher blood levels of glutamine (408 ± 25 μMin XRT +GLN vs 311 ± 19 μMin XRT −GLN,P< 0.05). In addition, provision of GLN maintained mucosal mass and reduced weight loss (P< 0.05). The data lend further support to the hypothesis that glutamine helps maintain the gut mucosal barrier and thereby decreases the incidence of bacterial translocation following bowel injury. The possible mechanisms by which glutamine may reduce the incidence of culture-positive mesenteric lymph nodes following abdominal XRT are discussed.