An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae.
An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae.
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DOI：
10.3791/61911
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发表时间：
2020-10-29
期刊：
影响因子：
--
通讯作者：
Galko MJ
中科院分区：
文献类型：
--
作者：
Lopez-Bellido R;Galko MJ
已发表的果蝇机械伤害感受试验导致了对行为的可变评估。在这里，我们制造了用于果蝇幼虫的定制金属镍钛合金（镍钛诺）细丝。这些机械探针类似于脊椎动物中用于测量机械伤害感受的von Frey细丝。在这里，我们演示了如何制作和校准这些机械探针，以及如何生成从阈下（无害或无害范围）到阈上（低到高有害范围）刺激的完整行为剂量反应。为了证明探针的实用性，我们研究了果蝇幼虫的组织损伤诱导的超敏反应。机械异常性疼痛（对正常无害的机械刺激的超敏反应）和痛觉过敏（对有害的机械刺激的过度反应）尚未在果蝇幼虫中建立。使用通常无害的机械探针或通常引起厌恶行为的探针，我们发现果蝇幼虫在组织损伤后发生机械过敏（异常性疼痛和痛觉过敏）。因此，我们在这里说明的机械探针和测定将可能是解剖机械超敏反应的基本分子/遗传机制的重要工具。
Published assays for mechanical nociception in Drosophila have led to variable assessments of behavior. Here, we fabricated, for use with Drosophila larvae, customized metal nickel-titanium alloy (nitinol) filaments. These mechanical probes are similar to the von Frey filaments used in vertebrates to measure mechanical nociception. Here, we demonstrate how to make and calibrate these mechanical probes and how to generate a full behavioral dose-response from subthreshold (innocuous or non-noxious range) to suprathreshold (low to high noxious range) stimuli. To demonstrate the utility of the probes, we investigated tissue damage-induced hypersensitivity in Drosophila larvae. Mechanical allodynia (hypersensitivity to a normally innocuous mechanical stimulus) and hyperalgesia (exaggerated responsiveness to a noxious mechanical stimulus) have not yet been established in Drosophila larvae. Using mechanical probes that are normally innocuous or probes that typically elicit an aversive behavior, we found that Drosophila larvae develop mechanical hypersensitization (both allodynia and hyperalgesia) after tissue damage. Thus, the mechanical probes and assay that we illustrate here will likely be important tools to dissect the fundamental molecular/genetic mechanisms of mechanical hypersensitivity.
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影响因子：
5.3
作者：
Lopez-Bellido, Roger;Puig, Stephanie;Gutstein, Howard B.
通讯作者：
Gutstein, Howard B.
影响因子：
9.2
作者：
Babcock, Daniel T.;Shi, Shanping;Jo, Juyeon;Shaw, Michael;Gutstein, Howard B.;Galko, Michael J.
通讯作者：
Galko, Michael J.
影响因子：
25
作者：
Hu C;Petersen M;Hoyer N;Spitzweck B;Tenedini F;Wang D;Gruschka A;Burchardt LS;Szpotowicz E;Schweizer M;Guntur AR;Yang CH;Soba P
通讯作者：
Soba P
影响因子：
5.3
作者：
Follansbee, Taylor L.;Gjelsvik, Kayla J.;Ganter, Geoffrey K.
通讯作者：
Ganter, Geoffrey K.
果蝇压电在机械伤害感受中的作用。
影响因子：
64.8
作者：
Kim SE;Coste B;Chadha A;Cook B;Patapoutian A
通讯作者：
Patapoutian A