Evaluation of Genetic Diversity in Wild Tachibana Population of Heda, Shizuoka, Using DNA Marker Analysis, and Stable Maintenance of the Population
Evaluation of Genetic Diversity in Wild Tachibana Population of Heda, Shizuoka, Using DNA Marker Analysis, and Stable Maintenance of the Population
复制标题
利用DNA标记分析评价静冈县河田野生橘种群遗传多样性及种群稳定维持
DOI：
10.2503/hrj.19.141
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Shigeyoshi Endo
中科院分区：
文献类型：
--
作者：
T. Shimizu;K. Nagakura;M. Okuda;Hirofumi Inagi;I. Okuda;Shigeyoshi Endo
橘（Citrus tachibana（Makino）Tanaka）是日本的一种柑橘属植物。早在奈良时代（~公元800年）就已被发现，从东海到九州岛的太平洋沿岸沿着的森林中有几个野生种群。虽然立花以前被认为是由一个单一的基因型，最近的研究与DNA标记表明，至少有三个不同的基因型。以前的工作是在不同的野生种群中进行的，但对每个种群内或种群之间的遗传多样性知之甚少。本研究以日本静冈县鹤田市的一个野生立花树种群（n = 64）为研究对象，其中伊达地区24株，神谷地区40株。使用DNA标记分析细胞核和细胞器基因组，我们证实，在总共60株植物（22在伊达和38在神谷）有立花-A株基因型。样品中4种未鉴定的柑橘属植物被基因分型为Natsudaidai（C. natsudaidai Hayata）。在这两个地区都没有发现自交或杂交后代。这些观察表明，从立花A获得杂交种作为种子亲本可能很困难，另一种自由授粉种子的DNA标记分析也显示了类似的结果。调查显示，一些年轻的立花树的人口，我们建议，无性繁殖的多胚种子可能有利于无性繁殖的立花人口。
Tachibana (Citrus tachibana (Makino) Tanaka) is a citrus species indigenous to Japan. It has been known since before the Nara period (~800 AD), and several wild populations are present in forests along the Pacific coast, from Tokai to Kyushu Island. Although tachibana was previously regarded as consisting of a single genotype, recent research with DNA markers suggested there are at least three different genotypes. Previous work was carried out across different wild populations, but little is known about the genetic diversity within each population, or among populations. In this study, wild tachibana trees of a population in Heda, Shizuoka (n = 64; 24 in Ida area and 40 in Kamiya area) were sampled to explore the population’s genetic diversity and promote utilization and conservation. Using DNA marker analysis for both nuclear and organelle genomes, we confirmed that, in total, 60 plants (22 in Ida and 38 in Kamiya) had a tachibana-A strain genotype. Four unidentified citrus plants in the sample were genotyped as Natsudaidai (C. natsudaidai Hayata). No selfed or hybrid offspring were found in either area. The observations suggest that it may be difficult to obtain hybrids from tachibana-A as seed parents, and another DNA marker analysis of open-pollinated seeds showed similar results. The survey revealed several young tachibana trees in the population, and we suggest that vegetative propagation by polyembryonic seeds may facilitate asexual maintenance of this tachibana population.
日本现代柑橘品种驯化和多样化过程的模型
DOI：
10.17660/actahortic.2019.1230.2
发表时间：
2019
期刊：
Acta Horticulturae
影响因子：
--
作者：
Shimizu T.;Kitajima A.;Nonaka K.;Yoshioka T.;Ohta S.;Goto S.;Kaminuma E.;Nakamura Y.
通讯作者：
Nakamura Y.