Lumbar lateral motor column development in triploid Xenopus laevis.
Lumbar lateral motor column development in triploid Xenopus laevis.
复制标题
三倍体非洲爪蟾的腰部横向运动柱发育。
DOI：
10.1002/cne.902780312
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Sperry,DG
中科院分区：
文献类型：
--
作者：
Sperry,DG
研究了增加倍性对非洲爪蟾腰椎外侧运动柱（L-LMC）发育的影响，以确定早期事件如何有助于产生细胞死亡后二倍体和三倍体动物中存在的运动神经元平均数量的显著差异（Sperry：J. Comp. Neurol. 277：499-508，'88）。从天然存在的二倍体和实验产生的三倍体同胞中，在显著量的神经元细胞死亡之前的两个阶段，在细胞死亡高峰期期间的一个阶段，以及在细胞死亡后的一个阶段，对L-LMC运动神经元进行计数，并测量核横截面积。在细胞死亡之前和之后的阶段，运动神经元和其他细胞的平均核横截面积也被测量在三倍体中更大，而运动神经元的平均数量和运动神经元密度（每节细胞的平均数量）则更小。二倍体和三倍体动物的平均体型和平均运动柱长度在每个阶段都是相等的。在三倍体动物中也观察到了在二倍体中广泛观察到的L-LMC发育的一般特征，即细胞大小增加伴细胞数量减少。然而，不仅是这些一般特征存在于三倍体，但平均运动神经元大小的增加和平均运动神经元数量的减少在二倍体和三倍体大致相等时，缩放到核大小的一般差异或运动神经元的平均数量的差异细胞死亡前。没有证据表明三倍体条件影响细胞死亡过程的程度。总体而言，研究结果表明，实验产生的三倍体中L-LMC发育的过程可能与自然产生的二倍体中的过程没有什么不同。细胞死亡后，在三倍体运动神经元的平均数较小，可能与一些功能的三倍体条件，影响细胞死亡前存在的运动神经元的数量，而不是一个功能的三倍体动物，如外周大小，这被认为是影响运动神经元细胞死亡过程本身。这些研究结果的一般问题的L-LMC运动神经元群体的大小在三倍体，以及在二倍体，可能会被调节的关系进行了讨论。
The effects of increasing ploidy on the development of the lumbar lateral motor column (L‐LMC) inXenopus laeviswere investigated in order to determine how early events contribute to producing the significant difference in the average number of motoneurons present in diploid and triploid animals after cell death (Sperry:J. Comp. Neurol. 277:499–508, '88). From naturally occurring diploid and experimentally produced triploid siblings at two stages prior to significant amounts of neuronal cell death, at one stage during the peak period of cell death, and at one stage after cell death, the L‐LMC motoneurons were counted and nuclear cross‐sectional areas were measured. At stages before and after cell death, the average nuclear crosssectional areas of motoneurons and of other cells that were also measured were greater in the triploids, while the average number of motoneurons and motoneuron density (the mean number of cells per section) were less. Average body size and average motor column length in diploid and triploid animals were equal at each of the stages. The general characteristics of L‐LMC development that have been widely noted in diploids, an increase in cell size accompanied by a decrease in cell number, were also observed in the triploid animals. However, not only were these general features present in the triploids, but the increase in average motoneuron size and the decrease in average motoneuron number in diploids and triploids were roughly equal when scaled to the general differences in nuclear size or to the difference in the average number of motoneurons present prior to cell death. There was no evidence that the triploid condition affected the magnitude of the cell death process.Overall, the findings suggest that the processes underlying L‐LMC development in experimentally produced triploids may not be different from those in naturally occurring diploids. The smaller average numbers of motoneurons after cell death in the triploids may be related to some feature of the triploid condition that affects the number of motoneurons present before cell death rather than to a feature of the triploid animals, such as peripheral size, that is thought to affect the motoneuron cell death process itself. The relationship of these findings to the general issue of how the size of the L‐LMC motoneuron population in triploids, as well as in diploids, might be regulated is discussed.
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