Inducible transcriptional activity of bcn-1 element from laminin gamma1-chain gene promoter in renal and nonrenal cells.
Inducible transcriptional activity of bcn-1 element from laminin gamma1-chain gene promoter in renal and nonrenal cells.
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肾细胞和非肾细胞中层粘连蛋白 γ1 链基因启动子的 bcn-1 元件的诱导转录活性。
DOI：
10.1152/ajprenal.1998.275.4.f518
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Bomsztyk,K
中科院分区：
文献类型：
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作者：
Suzuki,H;Denisenko,ON;Suzuki,Y;Schullery,DS;Bomsztyk,K
层粘连蛋白是细胞外基质的主要成分，其表达受生长因子调节。层粘连蛋白 γ1 链启动子包含一个新鉴定的转录元件，称为 bcn-1，它在系膜细胞中具有活性和可诱导性。在这项研究中，我们探索了其他肾细胞和非肾细胞中 bcn-1 元件的激活。在天然层粘连蛋白 γ1 链启动子的背景下或当克隆到异源启动子上游时，用佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA) 处理大鼠肾小球上皮细胞 (GEC) 会增加 bcn-1 转录元件的活性。用 PMA 处理 GEC 诱导核 DNA 结合活性 BCN-1，该活性在凝胶迁移测定中被 bcn-1 基序识别。这些结果证明 bcn-1 基序及其同源 BCN-1 因子可能调节 GEC 中层粘连蛋白 γ1 链的转录。 bcn-1 元件及其同源 BCN-1 DNA 结合活性也在猴肾 COS-7 和人 T 细胞 Jurkat 系中被诱导。来自 GEC、COS 和 Jurkat 细胞的原位紫外交联核蛋白的 SDS-PAGE 揭示了所有三种细胞系中存在一种主要的 110-115 kDa 加合物。这些结果证明 bcn-1 元件在来自不同哺乳动物物种的肾细胞和非肾细胞中具有活性，其中相同的蛋白质有助于诱导型 BCN-1 DNA 结合活性。
Laminin is a major component of the extracellular matrix whose expression is regulated by growth factors. The laminin γ1-chain promoter contains a newly identified transcriptional element denoted bcn-1 that is both active and inducible in mesangial cells. In this study, we explored activation of the bcn-1 element in other renal and nonrenal cells. Treatment of rat glomerular epithelial cells (GEC) with phorbol 12-myristate 13-acetate (PMA) increased activity of the bcn-1 transcriptional element, within the context of the native laminin γ1-chain promoter or when cloned upstream of a heterologous promoter. Treatment of GEC with PMA induced nuclear DNA-binding activity, BCN-1, which was recognized by the bcn-1 motif in a gel shift assay. These results provide evidence that the bcn-1 motif and its cognate BCN-1 factor(s) may regulate transcription of the laminin γ1-chain in GEC. The bcn-1 element and its cognate BCN-1 DNA-binding activity were also inducible in monkey kidney COS-7 and in human T cell Jurkat lines. SDS-PAGE of in situ ultraviolet cross-linked nucleoproteins from GEC, COS, and Jurkat cells revealed one major 110–115 kDa adduct in all three cell lines. These results demonstrate that the bcn-1 element is active in renal and nonrenal cells from different mammalian species where the same protein contributes to the inducible BCN-1 DNA-binding activity.