Biocatalytic fumarate synthesis from pyruvate and CO<sub>2</sub> as a feedstock
Biocatalytic fumarate synthesis from pyruvate and CO<sub>2</sub> as a feedstock
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以丙酮酸和CO<sub>2</sub>为原料生物催化合成富马酸
DOI：
10.1039/d2re00039c
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Amao Yutaka
中科院分区：
文献类型：
--
作者：
Takeuchi Mika;Amao Yutaka
富马酸盐是一种有用的不饱和二羧酸，用作不饱和聚酯树脂和多元醇的原料以及染料的媒染剂。富马酸由石油衍生的苯或丁烷作为起始原料合成，并且期望开发一种由可再生原料制备富马酸的方法。在这项工作中，生物催化合成富马酸从CO2和丙酮酸通过L-苹果酸作为中间体在水介质中使用的生物催化系统组成的苹果酸脱氢酶（草酰乙酸脱羧; ME）和脱氢酶（FUM）在NADH的存在下完成。在NADH存在下，ME和FUM体系25 h后丙酮酸转化为富马酸的转化率为14.4%。
Fumarate is a useful unsaturated dicarboxylic acid that is used as a raw material for unsaturated polyester resins and polyhydric alcohols and as a mordant for dyes. Fumaric acid is synthesized from petroleum-derived benzene or butane as a starting material, and it is desired to develop a method for preparing it from renewable raw materials. In this work, the biocatalytic synthesis of fumarate from CO2 and pyruvate viaL-malate as an intermediate in an aqueous medium using a biocatalytic system consisting of malate dehydrogenase (oxaloacetate-decarboxylating; ME) and fumarase (FUM) in the presence of NADH is accomplished. The conversion yield for pyruvate to fumarate with the system of ME and FUM in the presence of NADH was estimated to be 14.4% after 25 h.