A model system for analyzing intercellular communication through plasmodesmata using moss protonemata and leaves.
A model system for analyzing intercellular communication through plasmodesmata using moss protonemata and leaves.
复制标题
使用苔藓原丝和叶子分析胞间连丝的模型系统。
DOI：
10.1007/s10265-014-0690-7
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发表时间：
2015
影响因子：
2.8
通讯作者：
Tomomichi
中科院分区：
文献类型：
--
作者：
Kitagawa;Munenori and Fujita;Tomomichi
植物的生长、发育和环境反应需要对细胞间信号和营养物质的运动进行适当的调节。为此，植物有专门的细胞质通道，即胞间连丝(PD)，它允许相邻细胞之间的微分子和大分子的共质运动。内部和外部信号在时空上调节分子在PD中的运动，以控制植物的发育和环境响应。尽管分子靶向运动的某些方面已经被揭示，但分子通过PD的非靶向、可扩散流动的机制及其调节和功能仍然知之甚少，特别是在细胞水平上。以前，我们开发了一个系统来定量分析光能转换荧光蛋白Dendra2在单细胞水平上的非定向性移动，该组织位于青霉的丝状原丝状组织中。在原丝虫中，一维的细胞间通讯可以很容易地在细胞水平上观察和定量分析。在这篇综述中，我们描述了原丝虫是如何离开OFP的。专利可以用来研究通过PD的共质运动，并讨论这个系统如何帮助我们更好地理解PD在植物发育和环境响应中的调节和功能。
Plant growth, development, and environmental responses require the proper regulation of intercellular movement of signals and nutrients. For this, plants have specialized cytoplasmic channels, the plasmodesmata (PD), which allow the symplasmic movement of micro- and macromolecules between neighboring cells. Internal and external signals spatio-temporally regulate the movement of molecules through the PD to control plant development and environmental responses. Although some aspects of targeted movement of molecules have been revealed, the mechanisms of non-targeted, diffusible flow of molecules through PD, and its regulation and function, remain poorly understood, particularly at the cellular level. Previously, we developed a system to quantitatively analyze non-targeted movement of a photoconvertible fluorescent protein, Dendra2, at the single-cell level in the filamentous protonemata tissue of the mossPhyscomitrella patens. In protonemata, one-dimensional intercellular communication can be easily observed and quantitatively analyzed at the cellular level. In this review, we describe how protonemata and leaves ofP. patenscan be used to study symplasmic movement through PD, and discuss how this system can help improve our understanding of PD regulation and function in development and environmental responses in plants.