High-performance liquid chromatographic determination of theophylline and its derivatives with electrochemical detection.
High-performance liquid chromatographic determination of theophylline and its derivatives with electrochemical detection.
复制标题
电化学检测高效液相色谱法测定茶碱及其衍生物
DOI：
10.1016/0021-9673(75)85056-4
复制
发表时间：
1979
影响因子：
4.1
通讯作者：
W. J. Mayer
中科院分区：
文献类型：
--
作者：
M. Greenberg;W. J. Mayer
采用高效液相色谱- c8反相色谱柱-电化学检测法测定茶碱及其衍生物。分析物在1.24 V（相对于标准甘汞电极）下在蜡浸渍石墨电极上使用经典直流和电流采样直流模式氧化，并与254 nm紫外检测器的结果进行比较。极谱检测对茶碱的灵敏度高于紫外检测；此外，妨碍紫外线检测的干扰可以用极谱检测选择性地消除。
Theophylline and its derivatives are determined by high-performance liquid chromatographic separation on a C8reversed-phase column with electrochemical detection. The analyte is oxidized at 1.24 V (vs. the standard calomel electrode) on a wax-impregnated graphite electrode using classical d.c. and current sampled d.c. modes, and the results obtained compared with those of a 254 nm ultraviolet (UV) detector. Polarographic detection offers greater sensitivity for theophylline than UV detection; further, interferences which hamper UV detection may be selectively eliminated with polarographic detection.