EVIDENCE FOR INTRACISTRONIC COMPLEMENTATION OF THE PRODUCT OF THE INFLUENZA-VIRUS GENE PTRA (P3 OF FOWL PLAGUE VIRUS)
EVIDENCE FOR INTRACISTRONIC COMPLEMENTATION OF THE PRODUCT OF THE INFLUENZA-VIRUS GENE PTRA (P3 OF FOWL PLAGUE VIRUS)
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DOI：
10.1099/0022-1317-49-1-133
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发表时间：
1980-01-01
影响因子：
3.8
通讯作者：
SCHOLTISSEK, C
中科院分区：
文献类型：
--
作者：
HELLER, E;SCHOLTISSEK, C
禽瘟病毒（FPV）突变株tsI8和ts236的ts病变位于RNA片段2（Ptra基因，相当于P3）。在用这些突变体和ts 90或ts 93双重感染后，在非允许温度（40 ℃）下形成噬斑，ts 90或ts 93也在片段2中携带ts损伤。C）。这些噬斑不能在40 ℃下[在鸡胚细胞中]传代。C，并表现出不同于野生型病毒形成的形态。双重感染后感染性颗粒的产量显示出空斑数与稀释度之间的非线性相关性，表明感染细胞至少需要2个颗粒。所有的实验证据都指向P3蛋白质内的顺反子互补。
The ts lesion of the fowl plague virus (FPV) mutants ts I8 and ts 236 was located in RNA segment 2 (Ptra gene, corresponding to P3). After double-infection with these mutants and ts 90 or ts 93, which also carry a ts lesion in segment 2, plaques were formed at the non-permissive temperature (40.degree. C). These plaque cannot be passaged [in chick embryo cells] at 40.degree. C and exhibit a morphology which differs from those formed by the wild-type virus. The yield of infectious particles after double-infection shows a non-linear correlation between the plaque number and dilution, indicating that at least 2 particles are needed for infection of a cell. All experimental evidence points to an intracistronic complementation within the P3 protein.