Expression of chemokines and chemokine receptors in lesional and nonlesional upper skin of patients with atopic dermatitis
Expression of chemokines and chemokine receptors in lesional and nonlesional upper skin of patients with atopic dermatitis
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DOI：
10.1016/j.jaci.2009.07.004
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发表时间：
2009-10-01
影响因子：
14.2
通讯作者：
Novak, Natalija
中科院分区：
文献类型：
--
作者：
Gros, Eva;Bussmann, Caroline;Novak, Natalija
背景：树突状细胞 (DC) 亚型进出皮肤在特应性皮炎 (AD) 中发挥着关键作用。目的：我们试图确定表皮 DC 亚型的 CCR 模式和 CCL 表达与 AD 状态的关系。方法：在特应性斑贴试验前和 24 小时和 72 小时后从 AD 患者、慢性 AD 患者的皮肤、慢性 AD 患者的皮肤中采集剃须活检标本。牛皮癣和健康的皮肤。采用流式细胞术研究表皮DC的CCR表达，并通过实时PCR定量皮肤中chemo me mRNA水平。结果：应用过敏原后，CD1a(+)表皮DC总数增加，Langerin阳性CD1a(+) DC比例下降，Langerin阴性CD1a(+) DC比例增加。 Langerin阴性CD1a(+) DCs的CCR5和CCR6表达是急性AD的特征。与慢性 AD 患者相比，急性 AD 患者中 CCL1、CCL3、CCL4 和 CCL11 mRNA 的表达更高。只有 CCL，特别是 CCL1、CCL17 和 CCL18 的强烈增加伴随着湿疹的发展，并且与银屑病患者相比，CCL1、CCL13、CCL17 和 CCL18 表达的增加对于慢性 AD 患者具有特异性。 结论：在 AD 的急性期，DC 从真皮和血液前体中的招募和分化发生了改变。使用过敏原后 CCL 幅度的增加伴随着湿疹的发展。 （过敏临床免疫杂志 2009 年；124：753-60。）
Background: Trafficking of dendritic cell (DC) subtypes to and from the skin plays a pivotal role in atopic dermatitis (AD).Objectives: We sought to determine the CCR pattern of epidermal DC subtypes and CCL expression in relation to the state of AD.Methods: Shave biopsy specimens were taken from patients with AD before and after 24 and 72 hours of atopy patch testing and from the skin of patients with chronic AD, skin of patients with psoriasis, and healthy skin. CCR expression of epidermal DCs was studied by using flow cytometry, and chemo me mRNA levels in the skin were quantified by means of real-time PCR.Results: The total number of CD1a(+) epidermal DCs increased and the proportion of Langerin-positive CD1a(+) DCs decreased whereas the percentage of Langerin-negative CD1a(+) DCs increased after allergen application. Expression of CCR5 and CCR6 of Langerin-negative CD1a(+) DCs was characteristic for acute AD. Expression of CCL1 CCL3, CCL4, and CCL11 mRNA was greater in patients with acute AD versus that seen in patients with chronic AD. Only a strong increase of CCLs, in particular CCL1, CCL17, and CCL18, went along with eczema development, and increased CCL1, CCL13, CCL17 and CCL18 expression was specific for patients with chronic AD compared with those with psoriasis.Conclusion: Modified recruitment and differentiation of DCs from their dermal and blood precursors occurs in the acute phase of AD. A boost in the amplitude of CCLs after allergen application goes along with eczema development. (J Allergy Clin Immunol 2009;124:753-60.)